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| {{STRUCTURE_2bmo| PDB=2bmo | SCENE= }} | | {{STRUCTURE_2bmo| PDB=2bmo | SCENE= }} |
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| '''THE CRYSTAL STRUCTURE OF NITROBENZENE DIOXYGENASE'''
| | ===THE CRYSTAL STRUCTURE OF NITROBENZENE DIOXYGENASE=== |
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| ==Overview==
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| Nitroaromatic compounds are used extensively in many industrial processes and have been released into the environment where they are considered environmental pollutants. Nitroaromatic compounds, in general, are resistant to oxidative attack due to the electron-withdrawing nature of the nitro groups and the stability of the benzene ring. However, the bacterium Comamonas sp. strain JS765 can grow with nitrobenzene as a sole source of carbon, nitrogen and energy. Biodegradation is initiated by the nitrobenzene dioxygenase (NBDO) system. We have determined the structure of NBDO, which has a hetero-hexameric structure similar to that of several other Rieske non-heme iron dioxygenases. The catalytic subunit contains a Rieske iron-sulfur center and an active-site mononuclear iron atom. The structures of complexes with substrates nitrobenzene and 3-nitrotoluene reveal the structural basis for its activity with nitroarenes. The substrate pocket contains an asparagine residue that forms a hydrogen bond to the nitro-group of the substrate, and orients the substrate in relation to the active-site mononuclear iron atom, positioning the molecule for oxidation at the nitro-substituted carbon.
| | The line below this paragraph, {{ABSTRACT_PUBMED_15854650}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 15854650 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_15854650}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Rieske non-heme dioxygenase]] | | [[Category: Rieske non-heme dioxygenase]] |
| [[Category: Substrate specificity iron-sulfur]] | | [[Category: Substrate specificity iron-sulfur]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 20:30:15 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 15:33:05 2008'' |
