2f8x: Difference between revisions

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[[Image:2f8x.gif|left|200px]]<br />

[[Image:2f8x.gif|left|200px]]<br /><applet load="2f8x" size="350" color="white" frame="true" align="right" spinBox="true"

<applet load="2f8x" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

caption="2f8x, resolution 3.250Å" />

'''Crystal structure of activated Notch, CSL and MAML on HES-1 promoter DNA sequence'''<br />

==Overview==

Notch receptors transduce essential developmental signals between, neighboring cells by forming a complex that leads to transcription of, target genes upon activation. We report here the crystal structure of a, Notch transcriptional activation complex containing the ankyrin domain of, human Notch1 (ANK), the transcription factor CSL on cognate DNA, and a, polypeptide from the coactivator Mastermind-like-1 (MAML-1). Together, CSL, and ANK create a groove to bind the MAML-1 polypeptide as a kinked, 70 A, helix. The composite binding surface likely restricts the recruitment of, MAML proteins to promoters on which Notch:CSL complexes have been, preassembled, ensuring tight transcriptional control of Notch target, genes.

Notch receptors transduce essential developmental signals between neighboring cells by forming a complex that leads to transcription of target genes upon activation. We report here the crystal structure of a Notch transcriptional activation complex containing the ankyrin domain of human Notch1 (ANK), the transcription factor CSL on cognate DNA, and a polypeptide from the coactivator Mastermind-like-1 (MAML-1). Together, CSL and ANK create a groove to bind the MAML-1 polypeptide as a kinked, 70 A helix. The composite binding surface likely restricts the recruitment of MAML proteins to promoters on which Notch:CSL complexes have been preassembled, ensuring tight transcriptional control of Notch target genes.

==Disease==

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==About this Structure==

2F8X is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=2F8X OCA].

2F8X is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F8X OCA].

==Reference==

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[[Category: Homo sapiens]]

[[Category: Protein complex]]

[[Category: Blacklow, S.C.]]

[[Category: Blacklow, S C.]]

[[Category: Nam, Y.]]

[[Category: Sliz, P.]]

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[[Category: rel-homology region]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Mon Nov 12 22~~:01:~~27 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:18:55 2008''

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@@ Line 1: / Line 1: @@
-[[Image:2f8x.gif|left|200px]]<br />
+[[Image:2f8x.gif|left|200px]]<br /><applet load="2f8x" size="350" color="white" frame="true" align="right" spinBox="true"
-<applet load="2f8x" size="450" color="white" frame="true" align="right" spinBox="true"
 caption="2f8x, resolution 3.250&Aring;" />
 '''Crystal structure of activated Notch, CSL and MAML on HES-1 promoter DNA sequence'''<br />
 ==Overview==
-Notch receptors transduce essential developmental signals between, neighboring cells by forming a complex that leads to transcription of, target genes upon activation. We report here the crystal structure of a, Notch transcriptional activation complex containing the ankyrin domain of, human Notch1 (ANK), the transcription factor CSL on cognate DNA, and a, polypeptide from the coactivator Mastermind-like-1 (MAML-1). Together, CSL, and ANK create a groove to bind the MAML-1 polypeptide as a kinked, 70 A, helix. The composite binding surface likely restricts the recruitment of, MAML proteins to promoters on which Notch:CSL complexes have been, preassembled, ensuring tight transcriptional control of Notch target, genes.
+Notch receptors transduce essential developmental signals between neighboring cells by forming a complex that leads to transcription of target genes upon activation. We report here the crystal structure of a Notch transcriptional activation complex containing the ankyrin domain of human Notch1 (ANK), the transcription factor CSL on cognate DNA, and a polypeptide from the coactivator Mastermind-like-1 (MAML-1). Together, CSL and ANK create a groove to bind the MAML-1 polypeptide as a kinked, 70 A helix. The composite binding surface likely restricts the recruitment of MAML proteins to promoters on which Notch:CSL complexes have been preassembled, ensuring tight transcriptional control of Notch target genes.
 ==Disease==
@@ Line 11: / Line 10: @@
 ==About this Structure==
-F8X is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2F8X OCA].
+F8X is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F8X OCA].
 ==Reference==
@@ Line 17: / Line 16: @@
 [[Category: Homo sapiens]]
 [[Category: Protein complex]]
-[[Category: Blacklow, S.C.]]
+[[Category: Blacklow, S C.]]
 [[Category: Nam, Y.]]
 [[Category: Sliz, P.]]
@@ Line 27: / Line 26: @@
 [[Category: rel-homology region]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 22:01:27 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:18:55 2008''