2f9j: Difference between revisions

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[[Image:2f9j.gif|left|200px]]<br />

[[Image:2f9j.gif|left|200px]]<br /><applet load="2f9j" size="350" color="white" frame="true" align="right" spinBox="true"

<applet load="2f9j" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

caption="2f9j, resolution 3.00Å" />

'''3.0 angstrom resolution structure of a Y22M mutant of the spliceosomal protein p14 bound to a region of SF3b155'''<br />

==Overview==

The precise excision of introns from precursor mRNAs (pre-mRNAs) in, eukaryotes is accomplished by the spliceosome, a complex assembly, containing five small nuclear ribonucleoprotein (snRNP) particles. Human, p14, a component of the spliceosomal U2 and U11/U12 snRNPs, has been shown, to associate directly with the pre-mRNA branch adenosine early in, spliceosome assembly and within the fully assembled spliceosome. Here we, report the 2.5-A crystal structure of a complex containing p14 and a, peptide derived from the p14-associated U2 snRNP component SF3b155. p14, contains an RNA recognition motif (RRM), the surface of which is largely, occluded by a C-terminal alpha-helix and a portion of the SF3b155 peptide., An analysis of RNA.protein crosslinking to wild-type and mutant p14 shows, that the branch adenosine directly interacts with a conserved aromatic, within a pocket on the surface of the complex. This result, combined with, a comparison of the structure with known RRMs and pseudoRRMs as well as, model-building by using the electron cryomicroscopy structure of a, spliceosomal U11/U12 di-snRNP, suggests that p14.SF3b155 presents a, noncanonical surface for RNA recognition at the heart of the mammalian, spliceosome.

The precise excision of introns from precursor mRNAs (pre-mRNAs) in eukaryotes is accomplished by the spliceosome, a complex assembly containing five small nuclear ribonucleoprotein (snRNP) particles. Human p14, a component of the spliceosomal U2 and U11/U12 snRNPs, has been shown to associate directly with the pre-mRNA branch adenosine early in spliceosome assembly and within the fully assembled spliceosome. Here we report the 2.5-A crystal structure of a complex containing p14 and a peptide derived from the p14-associated U2 snRNP component SF3b155. p14 contains an RNA recognition motif (RRM), the surface of which is largely occluded by a C-terminal alpha-helix and a portion of the SF3b155 peptide. An analysis of RNA.protein crosslinking to wild-type and mutant p14 shows that the branch adenosine directly interacts with a conserved aromatic within a pocket on the surface of the complex. This result, combined with a comparison of the structure with known RRMs and pseudoRRMs as well as model-building by using the electron cryomicroscopy structure of a spliceosomal U11/U12 di-snRNP, suggests that p14.SF3b155 presents a noncanonical surface for RNA recognition at the heart of the mammalian spliceosome.

==About this Structure==

2F9J is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=2F9J OCA].

2F9J is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F9J OCA].

==Reference==

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[[Category: Homo sapiens]]

[[Category: Protein complex]]

[[Category: MacMillan, A.M.]]

[[Category: MacMillan, A M.]]

[[Category: Schellenberg, M.J.]]

[[Category: Schellenberg, M J.]]

[[Category: sf3bp14 sf3b155 sap155 p14y22m rrm]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Mon Nov 12 22~~:01:~~54 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:19:06 2008''

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@@ Line 1: / Line 1: @@
-[[Image:2f9j.gif|left|200px]]<br />
+[[Image:2f9j.gif|left|200px]]<br /><applet load="2f9j" size="350" color="white" frame="true" align="right" spinBox="true"
-<applet load="2f9j" size="450" color="white" frame="true" align="right" spinBox="true"
 caption="2f9j, resolution 3.00&Aring;" />
 '''3.0 angstrom resolution structure of a Y22M mutant of the spliceosomal protein p14 bound to a region of SF3b155'''<br />
 ==Overview==
-The precise excision of introns from precursor mRNAs (pre-mRNAs) in, eukaryotes is accomplished by the spliceosome, a complex assembly, containing five small nuclear ribonucleoprotein (snRNP) particles. Human, p14, a component of the spliceosomal U2 and U11/U12 snRNPs, has been shown, to associate directly with the pre-mRNA branch adenosine early in, spliceosome assembly and within the fully assembled spliceosome. Here we, report the 2.5-A crystal structure of a complex containing p14 and a, peptide derived from the p14-associated U2 snRNP component SF3b155. p14, contains an RNA recognition motif (RRM), the surface of which is largely, occluded by a C-terminal alpha-helix and a portion of the SF3b155 peptide., An analysis of RNA.protein crosslinking to wild-type and mutant p14 shows, that the branch adenosine directly interacts with a conserved aromatic, within a pocket on the surface of the complex. This result, combined with, a comparison of the structure with known RRMs and pseudoRRMs as well as, model-building by using the electron cryomicroscopy structure of a, spliceosomal U11/U12 di-snRNP, suggests that p14.SF3b155 presents a, noncanonical surface for RNA recognition at the heart of the mammalian, spliceosome.
+The precise excision of introns from precursor mRNAs (pre-mRNAs) in eukaryotes is accomplished by the spliceosome, a complex assembly containing five small nuclear ribonucleoprotein (snRNP) particles. Human p14, a component of the spliceosomal U2 and U11/U12 snRNPs, has been shown to associate directly with the pre-mRNA branch adenosine early in spliceosome assembly and within the fully assembled spliceosome. Here we report the 2.5-A crystal structure of a complex containing p14 and a peptide derived from the p14-associated U2 snRNP component SF3b155. p14 contains an RNA recognition motif (RRM), the surface of which is largely occluded by a C-terminal alpha-helix and a portion of the SF3b155 peptide. An analysis of RNA.protein crosslinking to wild-type and mutant p14 shows that the branch adenosine directly interacts with a conserved aromatic within a pocket on the surface of the complex. This result, combined with a comparison of the structure with known RRMs and pseudoRRMs as well as model-building by using the electron cryomicroscopy structure of a spliceosomal U11/U12 di-snRNP, suggests that p14.SF3b155 presents a noncanonical surface for RNA recognition at the heart of the mammalian spliceosome.
 ==About this Structure==
-F9J is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2F9J OCA].
+F9J is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F9J OCA].
 ==Reference==
@@ Line 14: / Line 13: @@
 [[Category: Homo sapiens]]
 [[Category: Protein complex]]
-[[Category: MacMillan, A.M.]]
+[[Category: MacMillan, A M.]]
-[[Category: Schellenberg, M.J.]]
+[[Category: Schellenberg, M J.]]
 [[Category: sf3bp14 sf3b155 sap155 p14y22m rrm]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 22:01:54 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:19:06 2008''