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| {{STRUCTURE_2bs8| PDB=2bs8 | SCENE= }} | | {{STRUCTURE_2bs8| PDB=2bs8 | SCENE= }} |
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| '''CRYSTAL STRUCTURE OF F17B-G IN COMPLEX WITH N-ACETYL-D-GLUCOSAMINE'''
| | ===CRYSTAL STRUCTURE OF F17B-G IN COMPLEX WITH N-ACETYL-D-GLUCOSAMINE=== |
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| ==Overview==
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| Since the introduction of structural genomics, the protein has been recognized as the most important variable in crystallization. Recent strategies to modify a protein to improve crystal quality have included rationally engineered point mutations, truncations, deletions and fusions. Five naturally occurring variants, differing in 1-18 amino acids, of the 177-residue lectin domain of the F17G fimbrial adhesin were expressed and purified in identical ways. For four out of the five variants crystals were obtained, mostly in non-isomorphous space groups, with diffraction limits ranging between 2.4 and 1.1 A resolution. A comparative analysis of the crystal-packing contacts revealed that the variable amino acids are often involved in lattice contacts and a single amino-acid substitution can suffice to radically change crystal packing. A statistical approach proved reliable to estimate the compatibilities of the variant sequences with the observed crystal forms. In conclusion, natural variation, universally present within prokaryotic species, is a valuable genetic resource that can be favourably employed to enhance the crystallization success rate with considerably less effort than other strategies.
| | The line below this paragraph, {{ABSTRACT_PUBMED_16041081}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 16041081 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_16041081}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Lectin]] | | [[Category: Lectin]] |
| [[Category: Protein-sugar complex]] | | [[Category: Protein-sugar complex]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 20:43:42 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 21:50:03 2008'' |
