2bt7: Difference between revisions

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[[Image:2bt7.gif|left|200px]]
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[[Image:2bt7.png|left|200px]]


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{{STRUCTURE_2bt7|  PDB=2bt7  |  SCENE=  }}  
{{STRUCTURE_2bt7|  PDB=2bt7  |  SCENE=  }}  


'''STRUCTURE OF THE C-TERMINAL RECEPTOR-BINDING DOMAIN OF AVIAN REOVIRUS FIBRE SIGMAC, CD CRYSTAL FORM'''
===STRUCTURE OF THE C-TERMINAL RECEPTOR-BINDING DOMAIN OF AVIAN REOVIRUS FIBRE SIGMAC, CD CRYSTAL FORM===




==Overview==
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Avian reovirus fibre, a homo-trimer of the sigmaC protein, is responsible for primary host cell attachment. The protein expressed in bacteria forms elongated fibres comprised of a carboxy-terminal globular head domain and a slender shaft, and partial proteolysis yielded a carboxy-terminal protease-stable domain that was amenable to crystallisation. Here, we show that this fragment retains receptor-binding capability and report its structure, solved using two-wavelength anomalous diffraction and refined using data collected from three different crystal forms at 2.1 angstroms, 2.35 angstroms and 3.0 angstroms resolution. The carboxy-terminal globular domain has a beta-barrel fold with the same overall topology as the mammalian reovirus fibre (sigma1). However, the monomers of the sigmaC trimer show a more splayed-out arrangement than in the sigma1 structure. Also resolved are two triple beta-spiral repeats of the shaft or stalk domain. The presence in the sequence of heptad repeats amino-terminal to these triple beta-spiral repeats suggests that the unresolved portion of the shaft domain contains a triple alpha-helical coiled-coil structure. Implications for the function and stability of the sigmaC protein are discussed.
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{{ABSTRACT_PUBMED_16236316}}


==About this Structure==
==About this Structure==
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[[Category: Triple beta-spiral]]
[[Category: Triple beta-spiral]]
[[Category: Viral protein]]
[[Category: Viral protein]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 20:45:54 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 20:12:46 2008''

Revision as of 17:12, 27 July 2008

File:2bt7.png

Template:STRUCTURE 2bt7

STRUCTURE OF THE C-TERMINAL RECEPTOR-BINDING DOMAIN OF AVIAN REOVIRUS FIBRE SIGMAC, CD CRYSTAL FORM

Template:ABSTRACT PUBMED 16236316

About this Structure

2BT7 is a Single protein structure of sequence from Avian orthoreovirus. Full crystallographic information is available from OCA.

Reference

Structure of the carboxy-terminal receptor-binding domain of avian reovirus fibre sigmaC., Guardado Calvo P, Fox GC, Hermo Parrado XL, Llamas-Saiz AL, Costas C, Martinez-Costas J, Benavente J, van Raaij MJ, J Mol Biol. 2005 Nov 18;354(1):137-49. Epub 2005 Sep 30. PMID:16236316

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