|
|
| Line 1: |
Line 1: |
| [[Image:2c08.gif|left|200px]] | | {{Seed}} |
| | [[Image:2c08.png|left|200px]] |
|
| |
|
| <!-- | | <!-- |
| Line 9: |
Line 10: |
| {{STRUCTURE_2c08| PDB=2c08 | SCENE= }} | | {{STRUCTURE_2c08| PDB=2c08 | SCENE= }} |
|
| |
|
| '''RAT ENDOPHILIN A1 BAR DOMAIN'''
| | ===RAT ENDOPHILIN A1 BAR DOMAIN=== |
|
| |
|
|
| |
|
| ==Overview==
| | <!-- |
| Endophilin-A1 is a BAR domain-containing protein enriched at synapses and is implicated in synaptic vesicle endocytosis. It binds to dynamin and synaptojanin via a C-terminal SH3 domain. We examine the mechanism by which the BAR domain and an N-terminal amphipathic helix, which folds upon membrane binding, work as a functional unit (the N-BAR domain) to promote dimerisation and membrane curvature generation. By electron paramagnetic resonance spectroscopy, we show that this amphipathic helix is peripherally bound in the plane of the membrane, with the midpoint of insertion aligned with the phosphate level of headgroups. This places the helix in an optimal position to effect membrane curvature generation. We solved the crystal structure of rat endophilin-A1 BAR domain and examined a distinctive insert protruding from the membrane interaction face. This insert is predicted to form an additional amphipathic helix and is important for curvature generation. Its presence defines an endophilin/nadrin subclass of BAR domains. We propose that N-BAR domains function as low-affinity dimers regulating binding partner recruitment to areas of high membrane curvature.
| | The line below this paragraph, {{ABSTRACT_PUBMED_16763559}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 16763559 is the PubMed ID number. |
| | --> |
| | {{ABSTRACT_PUBMED_16763559}} |
|
| |
|
| ==About this Structure== | | ==About this Structure== |
| Line 36: |
Line 40: |
| [[Category: Sh3 domain]] | | [[Category: Sh3 domain]] |
| [[Category: Transferase]] | | [[Category: Transferase]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 21:02:46 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 19:54:58 2008'' |
