2cbm: Difference between revisions

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[[Image:2cbm.gif|left|200px]]
{{Seed}}
[[Image:2cbm.png|left|200px]]


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{{STRUCTURE_2cbm|  PDB=2cbm  |  SCENE=  }}  
{{STRUCTURE_2cbm|  PDB=2cbm  |  SCENE=  }}  


'''CRYSTAL STRUCTURE OF THE APO-FORM OF A NEOCARZINOSTATIN MUTANT EVOLVED TO BIND TESTOSTERONE.'''
===CRYSTAL STRUCTURE OF THE APO-FORM OF A NEOCARZINOSTATIN MUTANT EVOLVED TO BIND TESTOSTERONE.===




==Overview==
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We have recently applied in vitro evolution methods to create in Neocarzinostatin a new binding site for a target molecule unrelated to its natural ligand. The main objective of this work was to solve the structure of some of the selected binders in complex with the target molecule: testosterone. Three proteins (1a.15, 3.24 and 4.1) were chosen as representative members of sequence families that came out of the selection process within different randomization schemes. In order to evaluate ligand-induced conformational adaptation, we also determined the structure of one of the proteins (3.24) in the free and complexed forms. Surprisingly, all these mutants bind not one but two molecules of testosterone in two very different ways. The 3.24 structure revealed that the protein spontaneously evolved in the system to bind two ligand molecules in one single binding crevice. These two binding sites are formed by substituted as well as by non-variable side-chains. The comparison with the free structure shows that only limited structural changes are observed upon ligand binding. The X-ray structures of the complex formed by 1a.15 and 4.1 Neocarzinostatin mutants revealed that the two variants form very similar dimers. These dimers were observed neither for the uncomplexed variants nor for wild-type Neocarzinostatin but were shown here to be induced by ligand binding. Comparison of the three complexed forms clearly suggests that these unanticipated structural responses resulted from the molecular arrangement used for the selection experiments.
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{{ABSTRACT_PUBMED_16529771}}


==About this Structure==
==About this Structure==
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[[Category: Dna-binding]]
[[Category: Dna-binding]]
[[Category: Phage-display]]
[[Category: Phage-display]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 21:39:31 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 13:36:30 2008''

Revision as of 10:36, 29 July 2008

File:2cbm.png

Template:STRUCTURE 2cbm

CRYSTAL STRUCTURE OF THE APO-FORM OF A NEOCARZINOSTATIN MUTANT EVOLVED TO BIND TESTOSTERONE.

Template:ABSTRACT PUBMED 16529771

About this Structure

2CBM is a Single protein structure of sequence from Streptomyces carzinostaticus. Full crystallographic information is available from OCA.

Reference

Structures of in vitro evolved binding sites on neocarzinostatin scaffold reveal unanticipated evolutionary pathways., Drevelle A, Graille M, Heyd B, Sorel I, Ulryck N, Pecorari F, Desmadril M, van Tilbeurgh H, Minard P, J Mol Biol. 2006 Apr 28;358(2):455-71. Epub 2006 Feb 20. PMID:16529771

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