2ccz: Difference between revisions

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[[Image:2ccz.gif|left|200px]]
{{Seed}}
[[Image:2ccz.png|left|200px]]


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{{STRUCTURE_2ccz|  PDB=2ccz  |  SCENE=  }}  
{{STRUCTURE_2ccz|  PDB=2ccz  |  SCENE=  }}  


'''CRYSTAL STRUCTURE OF E. COLI PRIMOSOMOL PROTEIN PRIB BOUND TO SSDNA'''
===CRYSTAL STRUCTURE OF E. COLI PRIMOSOMOL PROTEIN PRIB BOUND TO SSDNA===




==Overview==
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PriB is a primosomal protein required for replication restart in Escherichia coli. PriB stimulates PriA helicase activity via interaction with single-stranded DNA (ssDNA), but the molecular details of this interaction remain unclear. Here, we report the crystal structure of PriB complexed with a 15 bases oligonucleotide (dT15) at 2.7 A resolution. PriB shares structural similarity with the E.coli ssDNA-binding protein (EcoSSB). However, the structure of the PriB-dT15 complex reveals that PriB binds ssDNA differently. Results from filter-binding assays show that PriB-ssDNA interaction is salt-sensitive and cooperative. Mutational analysis suggests that the loop L45 plays an important role in ssDNA binding. Based on the crystal structure and biochemical analyses, we propose a cooperative mechanism for the binding of PriB to ssDNA and a model for the assembly of the PriA-PriB-ssDNA complex. This report presents the first structure of a replication restart primosomal protein complexed with DNA, and a novel model that explains the interactions between a dimeric oligonucleotide-binding-fold protein and ssDNA.
The line below this paragraph, {{ABSTRACT_PUBMED_16899446}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 16899446 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16899446}}


==About this Structure==
==About this Structure==
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[[Category: Wu, H N.]]
[[Category: Wu, H N.]]
[[Category: Primosome,prib,dna replication,dna repair,dna recombination,ssdna,single-stranded dna,dna/protein complex]]
[[Category: Primosome,prib,dna replication,dna repair,dna recombination,ssdna,single-stranded dna,dna/protein complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 07:15:38 2008''
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