2cdp: Difference between revisions

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[[Image:2cdp.gif|left|200px]]
{{Seed}}
[[Image:2cdp.png|left|200px]]


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{{STRUCTURE_2cdp|  PDB=2cdp  |  SCENE=  }}  
{{STRUCTURE_2cdp|  PDB=2cdp  |  SCENE=  }}  


'''STRUCTURE OF A CBM6 IN COMPLEX WITH NEOAGAROHEXAOSE'''
===STRUCTURE OF A CBM6 IN COMPLEX WITH NEOAGAROHEXAOSE===




==Overview==
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Carbohydrate recognition is central to the biological and industrial exploitation of plant structural polysaccharides. These insoluble polymers are recalcitrant to microbial degradation, and enzymes that catalyze this process generally contain non-catalytic carbohydrate binding modules (CBMs) that potentiate activity by increasing substrate binding. Agarose, a repeat of the disaccharide 3,6-anhydro-alpha-L-galactose-(1,3)-beta-D-galactopyranose-(1,4), is the dominant matrix polysaccharide in marine algae, yet the role of CBMs in the hydrolysis of this important polymer has not previously been explored. Here we show that family 6 CBMs, present in two different beta-agarases, bind specifically to the non-reducing end of agarose chains, recognizing only the first repeat of the disaccharide. The crystal structure of one of these modules Aga16B-CBM6-2, in complex with neoagarohexaose, reveals the mechanism by which the protein displays exquisite specificity, targeting the equatorial O4 and the axial O3 of the anhydro-L-galactose. Targeting of the CBM6 to the non-reducing end of agarose chains may direct the appended catalytic modules to areas of the plant cell wall attacked by beta-agarases where the matrix polysaccharide is likely to be more amenable to further enzymic hydrolysis.
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{{ABSTRACT_PUBMED_16601125}}


==About this Structure==
==About this Structure==
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[[Category: Carbohydrate-binding module]]
[[Category: Carbohydrate-binding module]]
[[Category: Hydrolase]]
[[Category: Hydrolase]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 21:53:34 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 08:09:45 2008''

Revision as of 05:09, 28 July 2008

File:2cdp.png

Template:STRUCTURE 2cdp

STRUCTURE OF A CBM6 IN COMPLEX WITH NEOAGAROHEXAOSE

Template:ABSTRACT PUBMED 16601125

About this Structure

2CDP is a Single protein structure of sequence from Saccharophagus degradans. Full crystallographic information is available from OCA.

Reference

Family 6 carbohydrate binding modules in beta-agarases display exquisite selectivity for the non-reducing termini of agarose chains., Henshaw J, Horne-Bitschy A, van Bueren AL, Money VA, Bolam DN, Czjzek M, Ekborg NA, Weiner RM, Hutcheson SW, Davies GJ, Boraston AB, Gilbert HJ, J Biol Chem. 2006 Jun 23;281(25):17099-107. Epub 2006 Apr 6. PMID:16601125

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