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| {{STRUCTURE_2ch9| PDB=2ch9 | SCENE= }} | | {{STRUCTURE_2ch9| PDB=2ch9 | SCENE= }} |
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| '''CRYSTAL STRUCTURE OF DIMERIC HUMAN CYSTATIN F'''
| | ===CRYSTAL STRUCTURE OF DIMERIC HUMAN CYSTATIN F=== |
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| ==Overview==
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| Cystatins are important natural cysteine protease inhibitors targeting primarily papain-like cysteine proteases, including cathepsins and parasitic proteases like cruzipain, but also mammalian asparaginyl endopeptidase. Mammalian cystatin F, which is expressed almost exclusively in hematopoietic cells and accumulates in lysosome-like organelles, has been implicated in the regulation of antigen presentation and other immune processes. It is an unusual cystatin superfamily member with a redox-regulated activation mechanism and a restricted specificity profile. We describe the 2.1A crystal structure of human cystatin F in its dimeric "off" state. The two monomers interact in a fashion not seen before for cystatins or cystatin-like proteins that is crucially dependent on an unusual intermolecular disulfide bridge, suggesting how reduction leads to monomer formation and activation. Strikingly, core sugars for one of the two N-linked glycosylation sites of cystatin F are well ordered, and their conformation and interactions with the protein indicate that this unique feature of cystatin F may modulate its inhibitory properties, in particular its reduced affinity toward asparaginyl endopeptidase compared with other cystatins.
| | The line below this paragraph, {{ABSTRACT_PUBMED_16601115}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 16601115 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_16601115}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: N-linked glycan]] | | [[Category: N-linked glycan]] |
| [[Category: Thiol protease inhibitor]] | | [[Category: Thiol protease inhibitor]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 22:08:03 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 22:43:27 2008'' |
