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| [[Image:2cuo.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_2cuo| PDB=2cuo | SCENE= }} | | {{STRUCTURE_2cuo| PDB=2cuo | SCENE= }} |
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| '''Collagen model peptide (PRO-PRO-GLY)9'''
| | ===Collagen model peptide (PRO-PRO-GLY)9=== |
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| ==Overview==
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| The crystal structure of a collagen-model peptide [(Pro-Pro-Gly)(9)](3) has been determined at 1.33 A resolution. Diffraction data were collected at 100 K using synchrotron radiation, which led to the first structural study of [(Pro-Pro-Gly)(n)](3) under cryogenic conditions. The crystals belong to the P2(1) space group with cell parameters of a = 25.95, b = 26.56, c = 80.14 Angstroms and beta = 90.0 degrees. The overall molecular conformation was consistent with the left-handed 7/2-helical model with an axial repeat of 20 A for native collagen. A total of 332 water molecules were found in an asymmetric unit. Proline residues in adjacent triple-helices exhibited three types of hydrophobic interactions. Furthermore, three types of hydrogen-bonding networks mediated by water molecules were observed between adjacent triple-helices. These hydrophobic interactions and hydrogen-bonding networks occurred at intervals of 20 Angstroms along the c-axis based on the previous sub-cell structures [(Pro-Pro-Gly)(n)](3) (n = 9, 10), which were also seen in the full-cell structure of [(Pro-Pro-Gly)(10)](3). Five proline residues at the Y position in the X-Y-Gly triplet were found in a down-puckering conformation, this being inconsistent with the recently proposed propensity-based hypothesis. These proline residues were forced to adopt opposing puckering because of the prevailing hydrophobic interaction between triple-helices compared with the Pro:Pro stacking interaction within a triple-helix. | | The line below this paragraph, {{ABSTRACT_PUBMED_16091587}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 16091587 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_16091587}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Puckering]] | | [[Category: Puckering]] |
| [[Category: Triple-helix]] | | [[Category: Triple-helix]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 23:06:40 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 01:05:15 2008'' |
