2cvq: Difference between revisions

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[[Image:2cvq.gif|left|200px]]
{{Seed}}
[[Image:2cvq.png|left|200px]]


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{{STRUCTURE_2cvq|  PDB=2cvq  |  SCENE=  }}  
{{STRUCTURE_2cvq|  PDB=2cvq  |  SCENE=  }}  


'''Crystal structure of NAD(H)-dependent malate dehydrogenase complexed with NADPH'''
===Crystal structure of NAD(H)-dependent malate dehydrogenase complexed with NADPH===




==Overview==
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For better understanding of the coenzyme specificity in NAD-dependent MDH (tMDH) from Thermus flavus AT-62, we determined the crystal structures of tMDH-NADP(H) complex at maximally 1.65 A resolution. The overall structure is almost the same as that of the tMDH-NADH complex. However, NADP(H) binds to tMDH in the reverse orientation, where adenine occupies the position near the catalytic center and nicotinamide is positioned at the adenine binding site of the tMDH-NADH complex. Consistent with this, kinetic analysis of the malate-oxidizing reaction revealed that NADP(+) inhibited tMDH at high concentrations. This has provided the first evidence for the alternative binding mode of the nicotinamide coenzyme, that has pseudo-symmetry in its structure, in a single enzyme.
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{{ABSTRACT_PUBMED_16009341}}


==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Crystal structure of NAD-dependent malate dehydrogenase complexed with NADP(H)., Tomita T, Fushinobu S, Kuzuyama T, Nishiyama M, Biochem Biophys Res Commun. 2005 Aug 26;334(2):613-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16009341 16009341]
Crystal structure of NAD-dependent malate dehydrogenase complexed with NADP(H)., Tomita T, Fushinobu S, Kuzuyama T, Nishiyama M, Biochem Biophys Res Commun. 2005 Aug 26;334(2):613-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16009341 16009341]
Determinants of protein thermostability observed in the 1.9-A crystal structure of malate dehydrogenase from the thermophilic bacterium Thermus flavus., Kelly CA, Nishiyama M, Ohnishi Y, Beppu T, Birktoft JJ, Biochemistry. 1993 Apr 20;32(15):3913-22. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8471603 8471603]
Preliminary X-ray diffraction analysis of a crystallizable mutant of malate dehydrogenase from the thermophile Thermus flavus., Kelly CA, Sarfaty S, Nishiyama M, Beppu T, Birktoft JJ, J Mol Biol. 1991 Sep 20;221(2):383-5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/1920425 1920425]
Refined crystal structure of cytoplasmic malate dehydrogenase at 2.5-A resolution., Birktoft JJ, Rhodes G, Banaszak LJ, Biochemistry. 1989 Jul 11;28(14):6065-81. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/2775751 2775751]
Nucleotide sequence of the malate dehydrogenase gene of Thermus flavus and its mutation directing an increase in enzyme activity., Nishiyama M, Matsubara N, Yamamoto K, Iijima S, Uozumi T, Beppu T, J Biol Chem. 1986 Oct 25;261(30):14178-83. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/3771528 3771528]
The presence of a histidine-aspartic acid pair in the active site of 2-hydroxyacid dehydrogenases. X-ray refinement of cytoplasmic malate dehydrogenase., Birktoft JJ, Banaszak LJ, J Biol Chem. 1983 Jan 10;258(1):472-82. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/6848515 6848515]
[[Category: Malate dehydrogenase]]
[[Category: Malate dehydrogenase]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Tomita, T.]]
[[Category: Tomita, T.]]
[[Category: Nad-dependent-mdh nadp+ complex]]
[[Category: Nad-dependent-mdh nadp+ complex]]
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