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| [[Image:2cwm.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_2cwm| PDB=2cwm | SCENE= }} | | {{STRUCTURE_2cwm| PDB=2cwm | SCENE= }} |
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| '''Native Crystal Structure of NO releasing inductive lectin from seeds of the Canavalia maritima (ConM)'''
| | ===Native Crystal Structure of NO releasing inductive lectin from seeds of the Canavalia maritima (ConM)=== |
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| ==Overview==
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| Here, we report the crystallographic study of a lectin from Canavalia maritima seeds (ConM) and its relaxant activity on vascular smooth muscle, to provide new insights into the understanding of structure/function relationships of this class of proteins. ConM was crystallized and its structure determined by standard molecular replacement techniques. The amino acid residues, previously suggested incorrectly by manual sequencing, have now been determined as I17, I53, S129, S134, G144, S164, P165, S187, V190, S169, T196, and S202. Analysis of the structure indicated a dimer in the asymmetric unit, two metal binding sites per monomer, and loops involved in the molecular oligomerization. These confer 98% similarity between ConM and other previously described lectins, derived from Canavalia ensiformis and Canavalia brasiliensis. Our functional data indicate that ConM exerts a concentration-dependent relaxant action on isolated aortic rings that probably occurs via an interaction with a specific lectin-binding site on the endothelium, resulting in a release of nitric oxide.
| | The line below this paragraph, {{ABSTRACT_PUBMED_16337811}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 16337811 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_16337811}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Lectin]] | | [[Category: Lectin]] |
| [[Category: X-ray diffraction]] | | [[Category: X-ray diffraction]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 23:13:34 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 01:14:16 2008'' |
