2d1u: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older editNewer edit →
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,330,058 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,330,058 edits
No edit summary
Line 1: Line 1:
[[Image:2d1u.gif|left|200px]]
{{Seed}}
[[Image:2d1u.png|left|200px]]


<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2d1u|  PDB=2d1u  |  SCENE=  }}  
{{STRUCTURE_2d1u|  PDB=2d1u  |  SCENE=  }}  


'''Solution strcuture of the periplasmic signaling domain of FecA from Escherichia coli'''
===Solution strcuture of the periplasmic signaling domain of FecA from Escherichia coli===




==Overview==
<!--  
Gram-negative bacteria possess outer membrane receptors that utilize energy provided by the TonB system to take up iron. Several of these receptors participate in extracytoplasmic factor (ECF) signalling through an N-terminal signalling domain that interacts with a periplasmic transmembrane anti-sigma factor protein and a cytoplasmic sigma factor protein. The structures of the intact TonB-dependent outer membrane receptor FecA from Escherichia coli and FpvA from Pseudomonas aeruginosa have recently been solved by protein crystallography; however, no electron density was detected for their periplasmic signalling domains, suggesting that it was either unfolded or flexible with respect to the remainder of the protein. Here we describe the well-defined solution structure of this domain solved by multidimensional nuclear magnetic resonance (NMR) spectroscopy. The monomeric protein construct contains the 79-residue N-terminal domain as well as the next 17 residues that are part of the receptor's plug domain. These form two clearly distinct regions: a highly structured domain at the N-terminal end followed by an extended flexible tail at the C-terminal end, which includes the 'TonB-box' region, and connects it to the plug domain of the receptor. The structured region consists of two alpha-helices that are positioned side by side and are sandwiched in between two small beta-sheets. This is a novel protein fold which appears to be preserved in all the periplasmic signalling domains of bacterial TonB-dependent outer membrane receptors that are involved in ECF signalling, because the hydrophobic residues that make up the core of the protein domain are highly conserved.
The line below this paragraph, {{ABSTRACT_PUBMED_16313612}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 16313612 is the PubMed ID number.
-->
{{ABSTRACT_PUBMED_16313612}}


==About this Structure==
==About this Structure==
2D1U is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D1U OCA].  
2D1U is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D1U OCA].  


==Reference==
==Reference==
Line 27: Line 31:
[[Category: Iron-uptake]]
[[Category: Iron-uptake]]
[[Category: Surface signaling]]
[[Category: Surface signaling]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 23:32:08 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 12:54:02 2008''

Revision as of 09:54, 29 July 2008

File:2d1u.png

Template:STRUCTURE 2d1u

Solution strcuture of the periplasmic signaling domain of FecA from Escherichia coli

Template:ABSTRACT PUBMED 16313612

About this Structure

2D1U is a Single protein structure of sequence from Escherichia coli. Full experimental information is available from OCA.

Reference

Nuclear magnetic resonance solution structure of the periplasmic signalling domain of the TonB-dependent outer membrane transporter FecA from Escherichia coli., Garcia-Herrero A, Vogel HJ, Mol Microbiol. 2005 Dec;58(5):1226-37. PMID:16313612

Page seeded by OCA on Tue Jul 29 12:54:02 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "https://proteopedia.org/index.php?title=2d1u&oldid=742029"