2dbl: Difference between revisions

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[[Image:2dbl.jpg|left|200px]]
{{Seed}}
[[Image:2dbl.png|left|200px]]


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{{STRUCTURE_2dbl|  PDB=2dbl  |  SCENE=  }}  
{{STRUCTURE_2dbl|  PDB=2dbl  |  SCENE=  }}  


'''MOLECULAR BASIS OF CROSS-REACTIVITY AND THE LIMITS OF ANTIBODY-ANTIGEN COMPLEMENTARITY'''
===MOLECULAR BASIS OF CROSS-REACTIVITY AND THE LIMITS OF ANTIBODY-ANTIGEN COMPLEMENTARITY===




==Overview==
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Two major unanswered questions concerning the specificity of antibodies are: how do structurally different antigens bind with high affinity to the same antibody, and what are the limits of the antibody combining site complementarity and flexibility that contribute to such crossreactivity? We report here a comparative analysis of the X-ray structures of five conformationally different steroids in complex with the Fab' fragment of an anti-progesterone antibody DB3 at 2.7 A. This antibody is unable to complement completely the shape of the hydrophobic antigen so that crossreactivity occurs with other ligands without major structural rearrangements of the binding site. Antigen specificity can be explained through conserved interactions of DB3 with the steroid D-ring, whereas some of the crossreactivity is realized through different binding orientations of the steroid skeleton that place the A-ring into alternative pockets on the antibody surface. The restricted gene usage of the VGAM3.8 family in the generation of anti-progesterone monoclonal antibodies may be explained by the specific interaction of VH hallmark residues with the steroid D-ring. This first detailed structure of steroid interactions with a protein could be applied to the understanding of general mechanisms of steroid recognition as well as in the design of specific binding sites for small hydrophobic ligands.
The line below this paragraph, {{ABSTRACT_PUBMED_8413674}}, adds the Publication Abstract to the page
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{{ABSTRACT_PUBMED_8413674}}


==About this Structure==
==About this Structure==
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[[Category: Wilson, I A.]]
[[Category: Wilson, I A.]]
[[Category: Immunoglobulin]]
[[Category: Immunoglobulin]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 00:06:38 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 10:06:40 2008''

Revision as of 07:06, 29 July 2008

File:2dbl.png

Template:STRUCTURE 2dbl

MOLECULAR BASIS OF CROSS-REACTIVITY AND THE LIMITS OF ANTIBODY-ANTIGEN COMPLEMENTARITY

Template:ABSTRACT PUBMED 8413674

About this Structure

2DBL is a Protein complex structure. Full crystallographic information is available from OCA.

Reference

Molecular basis of crossreactivity and the limits of antibody-antigen complementarity., Arevalo JH, Taussig MJ, Wilson IA, Nature. 1993 Oct 28;365(6449):859-63. PMID:8413674

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