2dep: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older editNewer edit →
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
No edit summary
Line 1: Line 1:
[[Image:2dep.jpg|left|200px]]
{{Seed}}
[[Image:2dep.png|left|200px]]


<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2dep|  PDB=2dep  |  SCENE=  }}  
{{STRUCTURE_2dep|  PDB=2dep  |  SCENE=  }}  


'''Crystal Structure of xylanase B from Clostridium stercorarium F9'''
===Crystal Structure of xylanase B from Clostridium stercorarium F9===


==Overview==
Recombinant mature xylanase B from Clostridium stercorarium has been prepared and crystallized by the sitting-drop vapour-diffusion method using 4 mg ml(-1) purified enzyme, 10.3%(w/v) polyethylene glycol 1500, 8.6%(v/v) glycerol and 0.34 M non-detergent sulfobetaine 195. A suitable crystal grew after incubation for ten weeks at 293 K. The crystal belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 64.76, b = 96.60, c = 138.44 A. X-ray diffraction data were collected to 1.80 A resolution.


==About this Structure==
==About this Structure==
2DEP is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_stercorarium Clostridium stercorarium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DEP OCA].  
2DEP is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Clostridium_stercorarium Clostridium stercorarium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DEP OCA].  


==Reference==
==Reference==
Crystallization and preliminary X-ray analysis of xylanase B from Clostridium stercorarium., Nishimoto M, Fushinobu S, Miyanaga A, Wakagi T, Shoun H, Sakka K, Ohmiya K, Nirasawa S, Kitaoka M, Hayashi K, Acta Crystallogr D Biol Crystallogr. 2004 Feb;60(Pt 2):342-3. Epub 2004, Jan 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14747719 14747719]
<ref group="xtra">PMID:14747719</ref><ref group="xtra">PMID:15914908</ref><ref group="xtra">PMID:16233324</ref><references group="xtra"/>
[[Category: Clostridium stercorarium]]
[[Category: Clostridium stercorarium]]
[[Category: Single protein]]
[[Category: Fushinobu, S.]]
[[Category: Fushinobu, S.]]
[[Category: Hayashi, K.]]
[[Category: Hayashi, K.]]
Line 33: Line 30:
[[Category: Structural genomic]]
[[Category: Structural genomic]]
[[Category: Xylan degradation]]
[[Category: Xylan degradation]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 00:17:07 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 04:49:41 2009''

Revision as of 02:49, 17 February 2009

File:2dep.png

Template:STRUCTURE 2dep

Crystal Structure of xylanase B from Clostridium stercorarium F9

About this Structure

2DEP is a 2 chains structure of sequences from Clostridium stercorarium. Full crystallographic information is available from OCA.

Reference

  1. Nishimoto M, Fushinobu S, Miyanaga A, Wakagi T, Shoun H, Sakka K, Ohmiya K, Nirasawa S, Kitaoka M, Hayashi K. Crystallization and preliminary X-ray analysis of xylanase B from Clostridium stercorarium. Acta Crystallogr D Biol Crystallogr. 2004 Feb;60(Pt 2):342-3. Epub 2004, Jan 23. PMID:14747719 doi:10.1107/S090744490302715X
  2. Nishimoto M, Kitaoka M, Fushinobu S, Hayashi K. The role of conserved arginine residue in loop 4 of glycoside hydrolase family 10 xylanases. Biosci Biotechnol Biochem. 2005 May;69(5):904-10. PMID:15914908
  3. Nishimoto M, Kitaoka M, Hayashi K. Employing chimeric xylanases to identify regions of an alkaline xylanase participating in enzyme activity at basic pH. J Biosci Bioeng. 2002;94(5):395-400. PMID:16233324

Page seeded by OCA on Tue Feb 17 04:49:41 2009

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "https://proteopedia.org/index.php?title=2dep&oldid=866325"