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[[Image:2dh1.gif|left|200px]]
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[[Image:2dh1.png|left|200px]]


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{{STRUCTURE_2dh1|  PDB=2dh1  |  SCENE=  }}  
{{STRUCTURE_2dh1|  PDB=2dh1  |  SCENE=  }}  


'''Crystal structure of peanut lectin lactose-azobenzene-4,4'-dicarboxylic acid-lactose complex'''
===Crystal structure of peanut lectin lactose-azobenzene-4,4'-dicarboxylic acid-lactose complex===


==Overview==
The x-ray crystal structure of the tetrameric T-antigen-binding lectin from peanut, M(r) 110,000, has been determined by using the multiple isomorphous replacement method and refined to an R value of 0.218 for 22,155 reflections within the 10- to 2.95-A resolution range. Each subunit has essentially the same characteristic tertiary fold that is found in other legume lectins. The structure, however, exhibits an unusual quaternary arrangement of subunits. Unlike other well-characterized tetrameric proteins with identical subunits, peanut lectin has neither 222 (D2) nor fourfold (C4) symmetry. A noncrystallographic twofold axis relates two halves of the molecule. The two monomers in each half are related by a local twofold axis. The mutual disposition of the axes is such that they do not lead to a closed point group. Furthermore, the structure of peanut lectin demonstrates that differences in subunit arrangement in legume lectins could be due to factors intrinsic to the protein molecule and, contrary to earlier suggestions, are not necessarily caused by interactions involving covalently linked sugar. The structure provides a useful framework for exploring the structural basis and the functional implications of the variability in the subunit arrangement in legume lectins despite all of them having nearly the same subunit structure, and also for investigating the general problem of "open" quaternary assembly in oligomeric proteins.


==About this Structure==
==About this Structure==
2DH1 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Arachis_hypogaea Arachis hypogaea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DH1 OCA].  
2DH1 is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Arachis_hypogaea Arachis hypogaea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DH1 OCA].  


==Reference==
==Reference==
Crystal structure of peanut lectin, a protein with an unusual quaternary structure., Banerjee R, Mande SC, Ganesh V, Das K, Dhanaraj V, Mahanta SK, Suguna K, Surolia A, Vijayan M, Proc Natl Acad Sci U S A. 1994 Jan 4;91(1):227-31. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8278370 8278370]
<ref group="xtra">PMID:8278370</ref><ref group="xtra">PMID:8656429</ref><ref group="xtra">PMID:14747696</ref><ref group="xtra">PMID:11878960</ref><references group="xtra"/>
[[Category: Arachis hypogaea]]
[[Category: Arachis hypogaea]]
[[Category: Single protein]]
[[Category: Jayaraman, N.]]
[[Category: Jayaraman, N.]]
[[Category: Natchiar, S K.]]
[[Category: Natchiar, S K.]]
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[[Category: Multivalency]]
[[Category: Multivalency]]
[[Category: Open quaternary structure]]
[[Category: Open quaternary structure]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 15:36:37 2009''

Revision as of 13:36, 17 February 2009

File:2dh1.png

Template:STRUCTURE 2dh1

Crystal structure of peanut lectin lactose-azobenzene-4,4'-dicarboxylic acid-lactose complex

About this Structure

2DH1 is a 4 chains structure of sequences from Arachis hypogaea. Full crystallographic information is available from OCA.

Reference

  1. Banerjee R, Mande SC, Ganesh V, Das K, Dhanaraj V, Mahanta SK, Suguna K, Surolia A, Vijayan M. Crystal structure of peanut lectin, a protein with an unusual quaternary structure. Proc Natl Acad Sci U S A. 1994 Jan 4;91(1):227-31. PMID:8278370
  2. Banerjee R, Das K, Ravishankar R, Suguna K, Surolia A, Vijayan M. Conformation, protein-carbohydrate interactions and a novel subunit association in the refined structure of peanut lectin-lactose complex. J Mol Biol. 1996 Jun 7;259(2):281-96. PMID:8656429 doi:10.1006/jmbi.1996.0319
  3. Kundhavai Natchiar S, Arockia Jeyaprakash A, Ramya TN, Thomas CJ, Suguna K, Surolia A, Vijayan M. Structural plasticity of peanut lectin: an X-ray analysis involving variation in pH, ligand binding and crystal structure. Acta Crystallogr D Biol Crystallogr. 2004 Feb;60(Pt 2):211-9. Epub 2004, Jan 23. PMID:14747696 doi:https://dx.doi.org/10.1107/S090744490302849X
  4. Srinivas O, Mitra N, Surolia A, Jayaraman N. Photoswitchable multivalent sugar ligands: synthesis, isomerization, and lectin binding studies of azobenzene-glycopyranoside derivatives. J Am Chem Soc. 2002 Mar 13;124(10):2124-5. PMID:11878960

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