2dhe: Difference between revisions

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[[Image:2dhe.jpg|left|200px]]
{{Seed}}
[[Image:2dhe.png|left|200px]]


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{{STRUCTURE_2dhe|  PDB=2dhe  |  SCENE=  }}  
{{STRUCTURE_2dhe|  PDB=2dhe  |  SCENE=  }}  


'''CRYSTALLOGRAPHIC ANALYSIS OF THE CATALYTIC MECHANISM OF HALOALKANE DEHALOGENASE'''
===CRYSTALLOGRAPHIC ANALYSIS OF THE CATALYTIC MECHANISM OF HALOALKANE DEHALOGENASE===




==Overview==
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Crystal structures of haloalkane dehalogenase were determined in the presence of the substrate 1,2-dichloroethane. At pH 5 and 4 degrees C, substrate is bound in the active site without being converted; warming to room temperature causes the substrate's carbon-chlorine bond to be broken, producing a chloride ion with concomitant alkylation of the active-site residue Asp124. At pH 6 and room temperature the alkylated enzyme is hydrolysed by a water molecule activated by the His289-Asp260 pair in the active site. These results show that catalysis by the dehalogenase proceeds by a two-step mechanism involving an ester intermediate covalently bound at Asp124.
The line below this paragraph, {{ABSTRACT_PUBMED_8515812}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 8515812 is the PubMed ID number.
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{{ABSTRACT_PUBMED_8515812}}


==About this Structure==
==About this Structure==
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[[Category: Verschueren, K H.G.]]
[[Category: Verschueren, K H.G.]]
[[Category: Dehalogenase]]
[[Category: Dehalogenase]]
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