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| [[Image:2dkv.jpg|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_2dkv| PDB=2dkv | SCENE= }} | | {{STRUCTURE_2dkv| PDB=2dkv | SCENE= }} |
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| '''Crystal structure of class I chitinase from Oryza sativa L. japonica'''
| | ===Crystal structure of class I chitinase from Oryza sativa L. japonica=== |
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| ==Overview==
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| Rice chitinases are encoded by a small multigene family. To clarify the overall organization of rice chitinase genes, we have isolated and characterized the genes Cht-1, Cht-2 and Cht-3. Although all the three genes encode class I chitinase, the nucleotide sequences of the coding regions of Cht-1 and Cht-3 are very similar (90%), while that of Cht-2 is clearly more divergent (78%). Only Cht-2 has a 130 bp intron and encodes a C-terminal peptide sequence similar to that known to function as a vacuolar targeting signal. In 5' flanking regions of Cht-1 and Cht-3, but not of Cht-2, conserved sequences (GGCCGGCYGCCCYAG) were found. Related sequences were found also in the 5' flanking regions of another chitinase gene and a beta-glucanase gene which has also been reported to be stress-induced in rice. RNA blot hybridization analysis demonstrated that the stress-induced expression patterns of the Cht-1 and Cht-3 genes are similar, but quite different from that of Cht-2. However, all three genes are active in unstressed roots. By restriction fragment length polymorphism (RFLP) linkage analysis, Cht-1 and Cht-3 were mapped onto chromosome 6 and shown to be closely linked (0.8 cM). Cht-2 was mapped onto chromosome 5. All these features suggest that the expression patterns of rice class I chitinase genes may be correlated with their levels of sequence divergence and their chromosomal location.
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| ==About this Structure== | | ==About this Structure== |
| 2DKV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Oryza_sativa Oryza sativa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DKV OCA]. | | 2DKV is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Oryza_sativa Oryza sativa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DKV OCA]. |
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| ==Reference== | | ==Reference== |
| Sequence variation, differential expression and chromosomal location of rice chitinase genes., Nishizawa Y, Kishimoto N, Saito A, Hibi T, Mol Gen Genet. 1993 Oct;241(1-2):1-10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7901749 7901749]
| | <ref group="xtra">PMID:7901749</ref><ref group="xtra">PMID:15327374</ref><references group="xtra"/> |
| [[Category: Chitinase]] | | [[Category: Chitinase]] |
| [[Category: Oryza sativa]] | | [[Category: Oryza sativa]] |
| [[Category: Single protein]]
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| [[Category: Kezuka, Y.]] | | [[Category: Kezuka, Y.]] |
| [[Category: Nishizawa, Y.]] | | [[Category: Nishizawa, Y.]] |
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| [[Category: Oryza sativa l. japonica]] | | [[Category: Oryza sativa l. japonica]] |
| [[Category: Whole structure]] | | [[Category: Whole structure]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 00:38:18 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 10:37:03 2009'' |
