2dm9: Difference between revisions

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[[Image:2dm9.gif|left|200px]]
{{Seed}}
[[Image:2dm9.png|left|200px]]


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{{STRUCTURE_2dm9|  PDB=2dm9  |  SCENE=  }}  
{{STRUCTURE_2dm9|  PDB=2dm9  |  SCENE=  }}  


'''Crystal Structure of PH1978 from Pyrococcus horikoshii OT3'''
===Crystal Structure of PH1978 from Pyrococcus horikoshii OT3===




==Overview==
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Archaeal H(+)-ATPase (A-ATPase) is composed of an A(1) region that hydrolyzes ATP and an integral membrane part A(0) that conducts protons. Subunit E is a component of peripheral stator(s) that physically links A(1) and A(0) parts of the A-ATPase. Here we report the first crystal structure of subunit E of A-ATPase from Pyrococcus horikoshii OT3 at 1.85 A resolution. The protomer structure of subunit E represents a novel fold. The quaternary structure of subunit E is a homodimer, which may constitute the core part of the stator. To investigate the relationship with other stator subunit H, the complex of subunits EH was prepared and characterized using electrophoresis, mass spectrometry, N-terminal sequencing and circular dichroism spectroscopy, which revealed the polymeric and highly helical nature of the EH complex with equimolar stoichiometry of both the subunits. On the basis of the modular architecture of stator subunits, it is suggested that both cytoplasm and membrane sides of the EH complex may interact with other subunits to link A(1) and A(0) parts.
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{{ABSTRACT_PUBMED_17189637}}


==About this Structure==
==About this Structure==
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[[Category: Rsgi]]
[[Category: Rsgi]]
[[Category: Structural genomic]]
[[Category: Structural genomic]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 00:43:49 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Sep 29 07:29:42 2008''

Revision as of 04:29, 29 September 2008

File:2dm9.png

Template:STRUCTURE 2dm9

Crystal Structure of PH1978 from Pyrococcus horikoshii OT3

Template:ABSTRACT PUBMED 17189637

About this Structure

2DM9 is a Single protein structure of sequence from Pyrococcus horikoshii. Full crystallographic information is available from OCA.

Reference

Dimeric core structure of modular stator subunit E of archaeal H+ -ATPase., Lokanath NK, Matsuura Y, Kuroishi C, Takahashi N, Kunishima N, J Mol Biol. 2007 Feb 23;366(3):933-44. Epub 2006 Dec 9. PMID:17189637

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