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| [[Image:2dq6.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_2dq6| PDB=2dq6 | SCENE= }} | | {{STRUCTURE_2dq6| PDB=2dq6 | SCENE= }} |
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| '''Crystal Structure of Aminopeptidase N from Escherichia coli'''
| | ===Crystal Structure of Aminopeptidase N from Escherichia coli=== |
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| ==Overview==
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| Aminopeptidase N from Escherichia coli is a broad specificity zinc exopeptidase belonging to aminopeptidase clan MA, family M1. The structures of the ligand-free form and the enzyme-bestatin complex were determined at 1.5- and 1.6-A resolution, respectively. The enzyme is composed of four domains: an N-terminal beta-domain (Met(1)-Asp(193)), a catalytic domain (Phe(194)-Gly(444)), a middle beta-domain (Thr(445)-Trp(546)), and a C-terminal alpha-domain (Ser(547)-Ala(870)). The structure of the catalytic domain exhibits similarity to thermolysin, and a metal-binding motif (HEXXHX(18)E) is found in the domain. The zinc ion is coordinated by His(297), His(301), Glu(320), and a water molecule. The groove on the catalytic domain that contains the active site is covered by the C-terminal alpha-domain, and a large cavity is formed inside the protein. However, there exists a small hole at the center of the C-terminal alpha-domain. The N terminus of bestatin is recognized by Glu(121) and Glu(264), which are located in the N-terminal and catalytic domains, respectively. Glu(298) and Tyr(381), located near the zinc ion, are considered to be involved in peptide cleavage. A difference revealed between the ligand-free form and the enzyme-bestatin complex indicated that Met(260) functions as a cushion to accept substrates with different N-terminal residue sizes, resulting in the broad substrate specificity of this enzyme.
| | The line below this paragraph, {{ABSTRACT_PUBMED_16885166}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 16885166 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_16885166}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Gluzincin metallopeptidase]] | | [[Category: Gluzincin metallopeptidase]] |
| [[Category: Zinc]] | | [[Category: Zinc]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 00:57:04 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 05:06:18 2008'' |
