2dqx: Difference between revisions

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[[Image:2dqx.jpg|left|200px]]
{{Seed}}
[[Image:2dqx.png|left|200px]]


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{{STRUCTURE_2dqx|  PDB=2dqx  |  SCENE=  }}  
{{STRUCTURE_2dqx|  PDB=2dqx  |  SCENE=  }}  


'''mutant beta-amylase (W55R) from soy bean'''
===mutant beta-amylase (W55R) from soy bean===




==Overview==
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Beta-amylase (EC 3.2.1.2) is starch-hydrolyzing exo-type enzyme that can catalyze the successive liberation of beta-maltose from the nonreducing ends of alpha-1,4-linked glucopyranosyl polymers. There is a well-known phenomenon called multiple or repetitive attack where the enzyme releases several maltose molecules in a single enzyme-substrate complex. In order to understand it further, we examined the beta-amylase-catalyzed reaction using maltooligosaccharides. The Monte Carlo method was applied for simulation of the beta-amylase-catalyzed reaction including the multiple attack mechanism. Through site-directed mutagenesis, we have successfully prepared a mutant enzyme which may be simulated as a multiple attack action reduced one with retaining significant hydrolytic activity. From the results of X-ray structure analysis of the mutant enzyme, it was clarified that one carboxyl residue plays a very important role in the multiple attack. The multiple attack action needs the force of enzyme sliding on the substrate. In addition, it is important for the multiple attack that the enzyme and substrate have the characteristics of a stable productive substrate-enzyme complex through a hydrogen bond between the nonreducing end of the substrate and the carboxyl residue of the enzyme.
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{{ABSTRACT_PUBMED_17223700}}


==About this Structure==
==About this Structure==
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[[Category: Ishikawa, K.]]
[[Category: Ishikawa, K.]]
[[Category: Amylase mutant sliding soy bean]]
[[Category: Amylase mutant sliding soy bean]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 00:59:07 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 13:22:01 2008''

Revision as of 10:22, 29 July 2008

File:2dqx.png

Template:STRUCTURE 2dqx

mutant beta-amylase (W55R) from soy bean

Template:ABSTRACT PUBMED 17223700

About this Structure

2DQX is a Single protein structure of sequence from Glycine max. Full crystallographic information is available from OCA.

Reference

Kinetic and structural analysis of enzyme sliding on a substrate: multiple attack in beta-amylase., Ishikawa K, Nakatani H, Katsuya Y, Fukazawa C, Biochemistry. 2007 Jan 23;46(3):792-8. PMID:17223700

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