2dt9: Difference between revisions

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[[Image:2dt9.jpg|left|200px]]
{{Seed}}
[[Image:2dt9.png|left|200px]]


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{{STRUCTURE_2dt9|  PDB=2dt9  |  SCENE=  }}  
{{STRUCTURE_2dt9|  PDB=2dt9  |  SCENE=  }}  


'''Crystal structure of the regulatory subunit of aspartate kinase from Thermus flavus'''
===Crystal structure of the regulatory subunit of aspartate kinase from Thermus flavus===


==Overview==
To reveal the catalytic mechanism of Thermus aspartate kinase, each of 29 amino acid residues that were highly conserved in the sequenced aspartate kinases, was replaced with alanine or leucine by PCR site-directed mutagenesis. Comparison of the kinetic parameters of these mutants with those of the wild-type aspartate kinase suggested that Thr47 was involved in binding aspartate and that Lys7 and Glu74 were involved in catalysis. Analysis of the effective concentrations of magnesium ion on the activity showed that the mutants with replacements at Ser41, Thr47, Asp154 and Asp182 required higher concentrations of magnesium ion. This suggests that these four residues play important roles in the binding of magnesium ions which are required for enzymatic activity.


==About this Structure==
==About this Structure==
2DT9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DT9 OCA].  
2DT9 is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DT9 OCA].  


==Reference==
==Reference==
Kinetic and mutation analyses of aspartate kinase from Thermus flavus., Kobashi N, Nishiyama M, Tanokura M, J Biosci Bioeng. 1999;87(6):739-45. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16232547 16232547]
<ref group="xtra">PMID:16232547</ref><ref group="xtra">PMID:7773416</ref><references group="xtra"/>
[[Category: Aspartate kinase]]
[[Category: Aspartate kinase]]
[[Category: Single protein]]
[[Category: Thermus thermophilus]]
[[Category: Thermus thermophilus]]
[[Category: Fushinobu, S.]]
[[Category: Fushinobu, S.]]
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[[Category: Protein-ligand complex]]
[[Category: Protein-ligand complex]]
[[Category: Regulatory subunit]]
[[Category: Regulatory subunit]]
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