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| {{STRUCTURE_2du9| PDB=2du9 | SCENE= }} | | {{STRUCTURE_2du9| PDB=2du9 | SCENE= }} |
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| '''crystal structure of the transcriptional factor from C.glutamicum'''
| | ===crystal structure of the transcriptional factor from C.glutamicum=== |
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| ==Overview==
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| Among the transcription factors, the helix-turn-helix (HTH) GntR family comprised of FadR, HutC, MocR, YtrA, AraR, and PlmA subfamilies regulates the most varied biological processes. Generally, proteins belonging to this family contain an N-terminal DNA-binding domain and a C-terminal effector-binding/oligomerization domain. The members of the YtrA subfamily are much shorter than other members of this family, with chain lengths of 120-130 residues with about 50 residues located in the C-terminal domain. Because of this length, the mode of dimerization and the ability to bind effectors by the C-terminal domain are puzzling. Here, we first report the structure of the transcription factor CGL2947 from Corynebacterium glutamicum, which belongs to the YtrA family. The monomer is composed of a DNA-binding domain containing a winged HTH motif in the N terminus and two helices (alpha4 and alpha5) with a fishhook-shaped arrangement in the C terminus. Helices alpha4 and alpha5 of two monomers intertwine together to form a novel homodimer assembly. The effector-accommodating pocket with 2-methyl-2,4-pentanediol (MPD) docked was located, and it was suggested to represent a novel mode of effector binding. The structures in two crystal forms (MPD-free and -bound in the proposed effector-binding pocket) were solved. The structural variations have implications regarding how the effector-induced conformational change modulates DNA affinity for YtrA family members.
| | The line below this paragraph, {{ABSTRACT_PUBMED_17766384}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 17766384 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_17766384}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Yao, M.]] | | [[Category: Yao, M.]] |
| [[Category: Winged helix-turn-helix]] | | [[Category: Winged helix-turn-helix]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 01:23:24 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 07:35:10 2008'' |
