2hkk: Difference between revisions
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New page: left|200px<br /> <applet load="2hkk" size="450" color="white" frame="true" align="right" spinBox="true" caption="2hkk, resolution 1.90Å" /> '''Carbonic anhydrase ... |
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[[Image:2hkk. | [[Image:2hkk.jpg|left|200px]]<br /><applet load="2hkk" size="350" color="white" frame="true" align="right" spinBox="true" | ||
<applet load="2hkk" size=" | |||
caption="2hkk, resolution 1.90Å" /> | caption="2hkk, resolution 1.90Å" /> | ||
'''Carbonic anhydrase activators: Solution and X-ray crystallography for the interaction of andrenaline with various carbonic anhydrase isoforms'''<br /> | '''Carbonic anhydrase activators: Solution and X-ray crystallography for the interaction of andrenaline with various carbonic anhydrase isoforms'''<br /> | ||
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==Overview== | ==Overview== | ||
The activation of the metalloenzyme carbonic anhydrase (CA, EC 4.2.1.1), with L-adrenaline and histamine has been investigated by kinetic and X-ray, crystallographic studies. L-Adrenaline behaves as a potent activator of, isozyme CA I (activation constant of 90 nM), being a much weaker activator, of isozyme CA II (activation constant of 96 microM). Isoforms CA IV, VA, VII, and XIV were activated by L-adrenaline with K(A)s in the range of, 36-63 microM. The X-ray crystal structure of the CA II-L-adrenaline adduct, revealed that the activator plugs the entrance of the active site cavity, obstructing it almost completely. | The activation of the metalloenzyme carbonic anhydrase (CA, EC 4.2.1.1), with L-adrenaline and histamine has been investigated by kinetic and X-ray, crystallographic studies. L-Adrenaline behaves as a potent activator of, isozyme CA I (activation constant of 90 nM), being a much weaker activator, of isozyme CA II (activation constant of 96 microM). Isoforms CA IV, VA, VII, and XIV were activated by L-adrenaline with K(A)s in the range of, 36-63 microM. The X-ray crystal structure of the CA II-L-adrenaline adduct, revealed that the activator plugs the entrance of the active site cavity, obstructing it almost completely. | ||
==About this Structure== | ==About this Structure== | ||
2HKK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with ZN, HG and ALE as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Carbonate_dehydratase Carbonate dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.1 4.2.1.1] Full crystallographic information is available from [http:// | 2HKK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=HG:'>HG</scene> and <scene name='pdbligand=ALE:'>ALE</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Carbonate_dehydratase Carbonate dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.1 4.2.1.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HKK OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: crystal structure]] | [[Category: crystal structure]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 15:11:30 2008'' | ||
