2hp8: Difference between revisions

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[[Image:2hp8.gif|left|200px]]<br />

[[Image:2hp8.gif|left|200px]]<br /><applet load="2hp8" size="350" color="white" frame="true" align="right" spinBox="true"

<applet load="2hp8" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

caption="2hp8" />

'''SOLUTION STRUCTURE OF HUMAN P8-MTCP1, A CYSTEINE-RICH PROTEIN ENCODED BY THE MTCP1 ONCOGENE,REVEALS A NEW ALPHA-HELICAL ASSEMBLY MOTIF, NMR, 30 STRUCTURES'''<br />

==Overview==

MTCP1 (for Mature-T-Cell Proliferation) is the first gene unequivocally, identified in the group of uncommon leukemias with a mature phenotype. The, three-dimensional solution structure of the human p8(MTCP1) protein, encoded by the MTCP1 oncogene was determined by homonuclear proton, two-dimensional NMR methods at 600 MHz. After sequence specific, assignments, a total of 931 distance restraints and 57 dihedral restraints, were collected. The location of the three previously unassigned disulfide, bridges was determined from preliminary DIANA structures, using a, statistical analysis of intercystinyl distances. The solution structure of, p8(MTCP1) is presented as a set of 30 DIANA structures, further refined by, restrained molecular dynamics using a simulated annealing protocol with, the AMBER force field. The r.m.s.d. values with respect to the mean, structure for the backbone and all heavy atoms for a family of 30, structures are 0.73(+/-0.28) and 1.17(+/-0.23) A, when the structured core, of the protein (residues 5 to 63) is considered. The solution structure of, p8(MTCP1) reveals an original scaffold consisting of three alpha helices, associated with a new cysteine motif. Two of the helices are covalently, paired by two disulfide bridges, forming an alpha-hairpin which resembles, an antiparallel coiled-coil. The third helix is oriented roughly parallel, to the plane defined by the alpha-antiparallel motif and its axis forms an, angle of approximately 60 degrees with respect to the main axis of this, motif.

MTCP1 (for Mature-T-Cell Proliferation) is the first gene unequivocally identified in the group of uncommon leukemias with a mature phenotype. The three-dimensional solution structure of the human p8(MTCP1) protein encoded by the MTCP1 oncogene was determined by homonuclear proton two-dimensional NMR methods at 600 MHz. After sequence specific assignments, a total of 931 distance restraints and 57 dihedral restraints were collected. The location of the three previously unassigned disulfide bridges was determined from preliminary DIANA structures, using a statistical analysis of intercystinyl distances. The solution structure of p8(MTCP1) is presented as a set of 30 DIANA structures, further refined by restrained molecular dynamics using a simulated annealing protocol with the AMBER force field. The r.m.s.d. values with respect to the mean structure for the backbone and all heavy atoms for a family of 30 structures are 0.73(+/-0.28) and 1.17(+/-0.23) A, when the structured core of the protein (residues 5 to 63) is considered. The solution structure of p8(MTCP1) reveals an original scaffold consisting of three alpha helices, associated with a new cysteine motif. Two of the helices are covalently paired by two disulfide bridges, forming an alpha-hairpin which resembles an antiparallel coiled-coil. The third helix is oriented roughly parallel to the plane defined by the alpha-antiparallel motif and its axis forms an angle of approximately 60 degrees with respect to the main axis of this motif.

==About this Structure==

2HP8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=2HP8 OCA].

2HP8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HP8 OCA].

==Reference==

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[[Category: Chiche, L.]]

[[Category: Roumestand, C.]]

[[Category: Strub, M.P.]]

[[Category: Strub, M P.]]

[[Category: cysteine motif]]

[[Category: hu-p8]]

[[Category: leukemia]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Mon Nov 12 22~~:34:~~47 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:44:12 2008''

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@@ Line 1: / Line 1: @@
-[[Image:2hp8.gif|left|200px]]<br />
+[[Image:2hp8.gif|left|200px]]<br /><applet load="2hp8" size="350" color="white" frame="true" align="right" spinBox="true"
-<applet load="2hp8" size="450" color="white" frame="true" align="right" spinBox="true"
 caption="2hp8" />
 '''SOLUTION STRUCTURE OF HUMAN P8-MTCP1, A CYSTEINE-RICH PROTEIN ENCODED BY THE MTCP1 ONCOGENE,REVEALS A NEW ALPHA-HELICAL ASSEMBLY MOTIF, NMR, 30 STRUCTURES'''<br />
 ==Overview==
-MTCP1 (for Mature-T-Cell Proliferation) is the first gene unequivocally, identified in the group of uncommon leukemias with a mature phenotype. The, three-dimensional solution structure of the human p8(MTCP1) protein, encoded by the MTCP1 oncogene was determined by homonuclear proton, two-dimensional NMR methods at 600 MHz. After sequence specific, assignments, a total of 931 distance restraints and 57 dihedral restraints, were collected. The location of the three previously unassigned disulfide, bridges was determined from preliminary DIANA structures, using a, statistical analysis of intercystinyl distances. The solution structure of, p8(MTCP1) is presented as a set of 30 DIANA structures, further refined by, restrained molecular dynamics using a simulated annealing protocol with, the AMBER force field. The r.m.s.d. values with respect to the mean, structure for the backbone and all heavy atoms for a family of 30, structures are 0.73(+/-0.28) and 1.17(+/-0.23) A, when the structured core, of the protein (residues 5 to 63) is considered. The solution structure of, p8(MTCP1) reveals an original scaffold consisting of three alpha helices, associated with a new cysteine motif. Two of the helices are covalently, paired by two disulfide bridges, forming an alpha-hairpin which resembles, an antiparallel coiled-coil. The third helix is oriented roughly parallel, to the plane defined by the alpha-antiparallel motif and its axis forms an, angle of approximately 60 degrees with respect to the main axis of this, motif.
+MTCP1 (for Mature-T-Cell Proliferation) is the first gene unequivocally identified in the group of uncommon leukemias with a mature phenotype. The three-dimensional solution structure of the human p8(MTCP1) protein encoded by the MTCP1 oncogene was determined by homonuclear proton two-dimensional NMR methods at 600 MHz. After sequence specific assignments, a total of 931 distance restraints and 57 dihedral restraints were collected. The location of the three previously unassigned disulfide bridges was determined from preliminary DIANA structures, using a statistical analysis of intercystinyl distances. The solution structure of p8(MTCP1) is presented as a set of 30 DIANA structures, further refined by restrained molecular dynamics using a simulated annealing protocol with the AMBER force field. The r.m.s.d. values with respect to the mean structure for the backbone and all heavy atoms for a family of 30 structures are 0.73(+/-0.28) and 1.17(+/-0.23) A, when the structured core of the protein (residues 5 to 63) is considered. The solution structure of p8(MTCP1) reveals an original scaffold consisting of three alpha helices, associated with a new cysteine motif. Two of the helices are covalently paired by two disulfide bridges, forming an alpha-hairpin which resembles an antiparallel coiled-coil. The third helix is oriented roughly parallel to the plane defined by the alpha-antiparallel motif and its axis forms an angle of approximately 60 degrees with respect to the main axis of this motif.
 ==About this Structure==
-HP8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2HP8 OCA].
+HP8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HP8 OCA].
 ==Reference==
@@ Line 17: / Line 16: @@
 [[Category: Chiche, L.]]
 [[Category: Roumestand, C.]]
-[[Category: Strub, M.P.]]
+[[Category: Strub, M P.]]
 [[Category: cysteine motif]]
 [[Category: hu-p8]]
 [[Category: leukemia]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 22:34:47 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:44:12 2008''