2hvz: Difference between revisions

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[[Image:2hvz.gif|left|200px]]<br />

[[Image:2hvz.gif|left|200px]]<br /><applet load="2hvz" size="350" color="white" frame="true" align="right" spinBox="true"

<applet load="2hvz" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

caption="2hvz" />

'''Solution structure of the RRM domain of SR rich factor 9G8'''<br />

==Overview==

The sequence-specific RNA-binding proteins SRp20 and 9G8 are the smallest, members of the serine- and arginine-rich (SR) protein family, well known, for their role in splicing. They also play a role in mRNA export, in, particular of histone mRNAs. We present the solution structures of the, free 9G8 and SRp20 RNA recognition motifs (RRMs) and of SRp20 RRM in, complex with the RNA sequence 5'CAUC3'. The SRp20-RNA structure reveals, that although all 4 nt are contacted by the RRM, only the 5' cytosine is, primarily recognized in a specific way. This might explain the numerous, consensus sequences found by SELEX (systematic evolution of ligands by, exponential enrichment) for the RRM of 9G8 and SRp20. Furthermore, we, identify a short arginine-rich peptide adjacent to the SRp20 and 9G8 RRMs, which does not contact RNA but is necessary and sufficient for interaction, with the export factor Tip-associated protein (TAP). Together, these, results provide a molecular description for mRNA and TAP recognition by, SRp20 and 9G8.

The sequence-specific RNA-binding proteins SRp20 and 9G8 are the smallest members of the serine- and arginine-rich (SR) protein family, well known for their role in splicing. They also play a role in mRNA export, in particular of histone mRNAs. We present the solution structures of the free 9G8 and SRp20 RNA recognition motifs (RRMs) and of SRp20 RRM in complex with the RNA sequence 5'CAUC3'. The SRp20-RNA structure reveals that although all 4 nt are contacted by the RRM, only the 5' cytosine is primarily recognized in a specific way. This might explain the numerous consensus sequences found by SELEX (systematic evolution of ligands by exponential enrichment) for the RRM of 9G8 and SRp20. Furthermore, we identify a short arginine-rich peptide adjacent to the SRp20 and 9G8 RRMs, which does not contact RNA but is necessary and sufficient for interaction with the export factor Tip-associated protein (TAP). Together, these results provide a molecular description for mRNA and TAP recognition by SRp20 and 9G8.

==About this Structure==

2HVZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=2HVZ OCA].

2HVZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HVZ OCA].

==Reference==

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Line 13:

[[Category: Homo sapiens]]

[[Category: Single protein]]

[[Category: Golovanov, A.P.]]

[[Category: Golovanov, A P.]]

[[Category: Hautbergue, G.M.]]

[[Category: Hautbergue, G M.]]

[[Category: Lian, L.Y.]]

[[Category: Lian, L Y.]]

[[Category: Tintaru, A.M.]]

[[Category: Tintaru, A M.]]

[[Category: Wilson, S.A.]]

[[Category: Wilson, S A.]]

[[Category: rrm]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Mon Nov 12 22~~:37:~~12 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:46:19 2008''

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@@ Line 1: / Line 1: @@
-[[Image:2hvz.gif|left|200px]]<br />
+[[Image:2hvz.gif|left|200px]]<br /><applet load="2hvz" size="350" color="white" frame="true" align="right" spinBox="true"
-<applet load="2hvz" size="450" color="white" frame="true" align="right" spinBox="true"
 caption="2hvz" />
 '''Solution structure of the RRM domain of SR rich factor 9G8'''<br />
 ==Overview==
-The sequence-specific RNA-binding proteins SRp20 and 9G8 are the smallest, members of the serine- and arginine-rich (SR) protein family, well known, for their role in splicing. They also play a role in mRNA export, in, particular of histone mRNAs. We present the solution structures of the, free 9G8 and SRp20 RNA recognition motifs (RRMs) and of SRp20 RRM in, complex with the RNA sequence 5'CAUC3'. The SRp20-RNA structure reveals, that although all 4 nt are contacted by the RRM, only the 5' cytosine is, primarily recognized in a specific way. This might explain the numerous, consensus sequences found by SELEX (systematic evolution of ligands by, exponential enrichment) for the RRM of 9G8 and SRp20. Furthermore, we, identify a short arginine-rich peptide adjacent to the SRp20 and 9G8 RRMs, which does not contact RNA but is necessary and sufficient for interaction, with the export factor Tip-associated protein (TAP). Together, these, results provide a molecular description for mRNA and TAP recognition by, SRp20 and 9G8.
+The sequence-specific RNA-binding proteins SRp20 and 9G8 are the smallest members of the serine- and arginine-rich (SR) protein family, well known for their role in splicing. They also play a role in mRNA export, in particular of histone mRNAs. We present the solution structures of the free 9G8 and SRp20 RNA recognition motifs (RRMs) and of SRp20 RRM in complex with the RNA sequence 5'CAUC3'. The SRp20-RNA structure reveals that although all 4 nt are contacted by the RRM, only the 5' cytosine is primarily recognized in a specific way. This might explain the numerous consensus sequences found by SELEX (systematic evolution of ligands by exponential enrichment) for the RRM of 9G8 and SRp20. Furthermore, we identify a short arginine-rich peptide adjacent to the SRp20 and 9G8 RRMs, which does not contact RNA but is necessary and sufficient for interaction with the export factor Tip-associated protein (TAP). Together, these results provide a molecular description for mRNA and TAP recognition by SRp20 and 9G8.
 ==About this Structure==
-HVZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2HVZ OCA].
+HVZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HVZ OCA].
 ==Reference==
@@ Line 14: / Line 13: @@
 [[Category: Homo sapiens]]
 [[Category: Single protein]]
-[[Category: Golovanov, A.P.]]
+[[Category: Golovanov, A P.]]
-[[Category: Hautbergue, G.M.]]
+[[Category: Hautbergue, G M.]]
-[[Category: Lian, L.Y.]]
+[[Category: Lian, L Y.]]
-[[Category: Tintaru, A.M.]]
+[[Category: Tintaru, A M.]]
-[[Category: Wilson, S.A.]]
+[[Category: Wilson, S A.]]
 [[Category: rrm]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 22:37:12 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:46:19 2008''