2ek8: Difference between revisions

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[[Image:2ek8.jpg|left|200px]]
{{Seed}}
[[Image:2ek8.png|left|200px]]


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{{STRUCTURE_2ek8|  PDB=2ek8  |  SCENE=  }}  
{{STRUCTURE_2ek8|  PDB=2ek8  |  SCENE=  }}  


'''Aminopeptidase from Aneurinibacillus sp. strain AM-1'''
===Aminopeptidase from Aneurinibacillus sp. strain AM-1===


==Overview==
To elucidate the structure and molecular mechanism of a characteristic proline-specific aminopeptidase produced by the thermophile Aneurinibacillus sp. strain AM-1, its gene was cloned and the recombinant protein was overexpressed in Escherichia coli, purified and crystallized using the hanging-drop vapour-diffusion method. X-ray diffraction data were collected to 1.8 A resolution from the recombinant aminopeptidase crystal. The crystals belong to the orthorhombic space group P2(1)2(1)2, with unit-cell parameters a = 93.62, b = 68.20, c = 76.84 A. A complete data set was also obtained from crystals of SeMet-substituted aminopeptidase. Data in the resolution range 20-2.1 A from the MAD data set from the SeMet-substituted crystal were used for phase determination.


==About this Structure==
==About this Structure==
2EK8 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacteria Bacteria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EK8 OCA].  
2EK8 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Bacteria Bacteria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EK8 OCA].  


==Reference==
==Reference==
Overexpression, purification, crystallization and preliminary X-ray crystallographic studies of a proline-specific aminopeptidase from Aneurinibacillus sp. strain AM-1., Akioka M, Nakano H, Horikiri A, Tsujimoto Y, Matsui H, Shimizu T, Nakatsu T, Kato H, Watanabe K, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Dec 1;62(Pt, 12):1266-8. Epub 2006 Nov 30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17142913 17142913]
<ref group="xtra">PMID:17142913</ref><references group="xtra"/>
[[Category: Bacteria]]
[[Category: Bacteria]]
[[Category: Single protein]]
[[Category: Akioka, M.]]
[[Category: Akioka, M.]]
[[Category: Nakano, H.]]
[[Category: Nakano, H.]]
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[[Category: Hydrolase]]
[[Category: Hydrolase]]
[[Category: Metalloproteinase]]
[[Category: Metalloproteinase]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 02:40:23 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 11:43:26 2009''

Revision as of 09:43, 16 February 2009

File:2ek8.png

Template:STRUCTURE 2ek8

Aminopeptidase from Aneurinibacillus sp. strain AM-1

About this Structure

2EK8 is a 1 chain structure of sequence from Bacteria. Full crystallographic information is available from OCA.

Reference

  1. Akioka M, Nakano H, Horikiri A, Tsujimoto Y, Matsui H, Shimizu T, Nakatsu T, Kato H, Watanabe K. Overexpression, purification, crystallization and preliminary X-ray crystallographic studies of a proline-specific aminopeptidase from Aneurinibacillus sp. strain AM-1. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Dec 1;62(Pt, 12):1266-8. Epub 2006 Nov 30. PMID:17142913 doi:10.1107/S1744309106047543

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