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[[Image:2enx.jpg|left|200px]]
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[[Image:2enx.png|left|200px]]


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{{STRUCTURE_2enx|  PDB=2enx  |  SCENE=  }}  
{{STRUCTURE_2enx|  PDB=2enx  |  SCENE=  }}  


'''Structure of the family II inorganic pyrophosphatase from Streptococcus agalactiae at 2.8 resolution'''
===Structure of the family II inorganic pyrophosphatase from Streptococcus agalactiae at 2.8 resolution===




==Overview==
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Streptococcus agalactiae, a prokaryote that causes infections in neonates and immunocompromised adults, has a serine/threonine protein kinase (STK) signalling cascade. The structure of one of the targets, a family II inorganic pyrophosphatase, has been solved by molecular replacement and refined at 2.80 A resolution to an R factor of 19.2% (R(free) = 26.7%). The two monomers in the asymmetric unit are related by a noncrystallographic twofold axis, but the biological dimer is formed by a crystallographic twofold. Each monomer contains the pyrophosphate analogue imidodiphosphate (PNP) and three metal ions per active site: two Mn(2+) ions in sites M1 and M2 and an Mg(2+) ion in site M3. The enzyme is in the closed conformation. Like other family II enzymes, the structure consists of two domains (residues 1-191 and 198-311), with the active site located between them. The conformation of Lys298 in the active site is different from those observed previously and it coordinates to the conserved DHH motif in a unique way. The structure suggests that Ser150, Ser194, Ser195 and Ser296 are the most likely targets for the Ser/Thr kinase and phosphatase because they are surface-accessible and either in the active site or in the hinge region between the two domains.
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{{ABSTRACT_PUBMED_17505113}}


==About this Structure==
==About this Structure==
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[[Category: Signalling]]
[[Category: Signalling]]
[[Category: Streptococcus agalactiae]]
[[Category: Streptococcus agalactiae]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 00:04:50 2008''

Revision as of 21:04, 28 July 2008

File:2enx.png

Template:STRUCTURE 2enx

Structure of the family II inorganic pyrophosphatase from Streptococcus agalactiae at 2.8 resolution

Template:ABSTRACT PUBMED 17505113

About this Structure

2ENX is a Single protein structure of sequence from Streptococcus agalactiae. Full crystallographic information is available from OCA.

Reference

Structure of the Streptococcus agalactiae family II inorganic pyrophosphatase at 2.80 A resolution., Rantanen MK, Lehtio L, Rajagopal L, Rubens CE, Goldman A, Acta Crystallogr D Biol Crystallogr. 2007 Jun;63(Pt 6):738-43. Epub 2007, May 15. PMID:17505113

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