2evi: Difference between revisions

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[[Image:2evi.gif|left|200px]]
{{Seed}}
[[Image:2evi.png|left|200px]]


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{{STRUCTURE_2evi|  PDB=2evi  |  SCENE=  }}  
{{STRUCTURE_2evi|  PDB=2evi  |  SCENE=  }}  


'''Structure of a Ndt80-DNA complex (MSE mutant mA8T)'''
===Structure of a Ndt80-DNA complex (MSE mutant mA8T)===




==Overview==
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The Saccharomyces cerevisiae transcription factor Ndt80 selectively binds a DNA consensus sequence (the middle sporulation element [MSE]) to activate gene expression after the successful completion of meiotic recombination. Here we report the X-ray crystal structures of Ndt80 bound to ten distinct MSE variants. Comparison of these structures with the structure of Ndt80 bound to a consensus MSE reveals structural principles that determine the DNA binding specificity of this transcription factor. The 5' GC-rich end of the MSE contains distinct 5'-YpG-3' steps that are recognized by arginine side chains through a combination of hydrogen bonding and cation-pi interactions. The 3' AT-rich region is recognized via minor groove contacts that sterically exclude the N2 atom of GC base pairs. The conformation of the AT-rich region is fixed by interactions with the protein that favor recognition of poly(A)-poly(T) versus mixed AT sequences through an avoidance of major groove steric clashes at 5'-ApT-3' steps.
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{{ABSTRACT_PUBMED_16531239}}


==About this Structure==
==About this Structure==
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[[Category: Beta-barrel]]
[[Category: Beta-barrel]]
[[Category: Ig-fold transcription factor]]
[[Category: Ig-fold transcription factor]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 03:09:38 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 10:48:22 2008''

Revision as of 07:48, 28 July 2008

File:2evi.png

Template:STRUCTURE 2evi

Structure of a Ndt80-DNA complex (MSE mutant mA8T)

Template:ABSTRACT PUBMED 16531239

About this Structure

2EVI is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

Principles of protein-DNA recognition revealed in the structural analysis of Ndt80-MSE DNA complexes., Lamoureux JS, Glover JN, Structure. 2006 Mar;14(3):555-65. PMID:16531239

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