2ez9: Difference between revisions

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[[Image:2ez9.gif|left|200px]]
{{Seed}}
[[Image:2ez9.png|left|200px]]


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{{STRUCTURE_2ez9|  PDB=2ez9  |  SCENE=  }}  
{{STRUCTURE_2ez9|  PDB=2ez9  |  SCENE=  }}  


'''Pyruvate oxidase variant F479W in complex with reaction intermediate analogue 2-phosphonolactyl-thiamin diphosphate'''
===Pyruvate oxidase variant F479W in complex with reaction intermediate analogue 2-phosphonolactyl-thiamin diphosphate===




==Overview==
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Enzymes that use the cofactor thiamin diphosphate (ThDP, 1), the biologically active form of vitamin B(1), are involved in numerous metabolic pathways in all organisms. Although a theory of the cofactor's underlying reaction mechanism has been established over the last five decades, the three-dimensional structures of most major reaction intermediates of ThDP enzymes have remained elusive. Here, we report the X-ray structures of key intermediates in the oxidative decarboxylation of pyruvate, a central reaction in carbon metabolism catalyzed by the ThDP- and flavin-dependent enzyme pyruvate oxidase (POX)3 from Lactobacillus plantarum. The structures of 2-lactyl-ThDP (LThDP, 2) and its stable phosphonate analog, of 2-hydroxyethyl-ThDP (HEThDP, 3) enamine and of 2-acetyl-ThDP (AcThDP, 4; all shown bound to the enzyme's active site) provide profound insights into the chemical mechanisms and the stereochemical course of thiamin catalysis. These snapshots also suggest a mechanism for a phosphate-linked acyl transfer coupled to electron transfer in a radical reaction of pyruvate oxidase.
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{{ABSTRACT_PUBMED_16680160}}


==About this Structure==
==About this Structure==
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[[Category: Reaction intermediate analogue]]
[[Category: Reaction intermediate analogue]]
[[Category: Tpp enzyme]]
[[Category: Tpp enzyme]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 03:16:42 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 19:34:25 2008''

Revision as of 16:34, 28 July 2008

File:2ez9.png

Template:STRUCTURE 2ez9

Pyruvate oxidase variant F479W in complex with reaction intermediate analogue 2-phosphonolactyl-thiamin diphosphate

Template:ABSTRACT PUBMED 16680160

About this Structure

2EZ9 is a Single protein structure of sequence from Lactobacillus plantarum. Full crystallographic information is available from OCA.

Reference

The catalytic cycle of a thiamin diphosphate enzyme examined by cryocrystallography., Wille G, Meyer D, Steinmetz A, Hinze E, Golbik R, Tittmann K, Nat Chem Biol. 2006 Jun;2(6):324-8. Epub 2006 May 7. PMID:16680160

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