2ezi: Difference between revisions

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[[Image:2ezi.gif|left|200px]]
{{Seed}}
[[Image:2ezi.png|left|200px]]


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{{STRUCTURE_2ezi|  PDB=2ezi  |  SCENE=  }}  
{{STRUCTURE_2ezi|  PDB=2ezi  |  SCENE=  }}  


'''SOLUTION NMR STRUCTURE OF THE IGAMMA SUBDOMAIN OF THE MU END DNA BINDING DOMAIN OF MU PHAGE TRANSPOSASE, 30 STRUCTURES'''
===SOLUTION NMR STRUCTURE OF THE IGAMMA SUBDOMAIN OF THE MU END DNA BINDING DOMAIN OF MU PHAGE TRANSPOSASE, 30 STRUCTURES===




==Overview==
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The MuA transposase of phase Mu is a large modular protein that plays a central role in transposition. We show that the Mu end DNA-binding domain, I beta gamma, which is responsible for binding the DNA attachment sites at each end of the Mu genome, comprises two subdomains, I beta and I gamma, that are structurally autonomous and do not interact with each other in the absence of DNA. The solution structure of the I gamma subdomain has been determined by multidimensional NMR spectroscopy. The structure of I gamma comprises a four helix bundle and, despite the absence of any significant sequence identity, the topology of the first three helices is very similar to that of the homeodomain family of helix-turn-helix DNA-binding proteins. The helix-turn-helix motif of I gamma, however, differs from that of the homeodomains in so far as the loop is longer and the second helix is shorter, reminiscent of that in the POU-specific domain.
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{{ABSTRACT_PUBMED_9367742}}


==About this Structure==
==About this Structure==
2EZI is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_mu Enterobacteria phage mu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EZI OCA].  
2EZI is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_mu Enterobacteria phage mu]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EZI OCA].  


==Reference==
==Reference==
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[[Category: Dna-binding protein]]
[[Category: Dna-binding protein]]
[[Category: Transposition]]
[[Category: Transposition]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 03:17:20 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 17:45:02 2008''

Revision as of 14:45, 27 July 2008

File:2ezi.png

Template:STRUCTURE 2ezi

SOLUTION NMR STRUCTURE OF THE IGAMMA SUBDOMAIN OF THE MU END DNA BINDING DOMAIN OF MU PHAGE TRANSPOSASE, 30 STRUCTURES

Template:ABSTRACT PUBMED 9367742

About this Structure

2EZI is a Single protein structure of sequence from Enterobacteria phage mu. Full experimental information is available from OCA.

Reference

Solution structure of the I gamma subdomain of the Mu end DNA-binding domain of phage Mu transposase., Clubb RT, Schumacher S, Mizuuchi K, Gronenborn AM, Clore GM, J Mol Biol. 1997 Oct 17;273(1):19-25. PMID:9367742

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