2f2a: Difference between revisions

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[[Image:2f2a.~~gif~~|left|200px]]

[[Image:2f2a.png|left|200px]]

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~~'''~~Structure of tRNA-Dependent Amidotransferase GatCAB complexed with Gln~~'''~~

===Structure of tRNA-Dependent Amidotransferase GatCAB complexed with Gln===

~~==Overview==~~

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The ~~formation of glutaminyl transfer RNA (Gln-tRNA(Gln)) differs among~~ the ~~three domains of life. Most bacteria employ an indirect pathway~~ to ~~produce Gln-tRNA~~(~~Gln) by a heterotrimeric glutamine amidotransferase CAB (GatCAB) that acts~~ on ~~the misacylated Glu-tRNA(Gln~~)~~. Here~~, ~~we describe a series of crystal structures of intact GatCAB from Staphylococcus aureus in~~ the ~~apo form and in the complexes with glutamine, asparagine, Mn2+, and adenosine triphosphate analog~~. ~~Two identified catalytic centers for the glutaminase and transamidase reactions are markedly distant but connected by a hydrophilic ammonia channel 30 A in length. Further, we show that the first U~~-A base pair in the acceptor stem and the D loop of tRNA(Gln) serve as identity elements essential for discrimination by GatCAB and propose a complete model for the overall concerted reactions to synthesize Gln-~~tRNA(Gln).~~

The line below this paragraph, {{ABSTRACT_PUBMED_16809541}}, adds the Publication Abstract to the page

(as it appears on PubMed at http://www.pubmed.gov), where 16809541 is the PubMed ID number.

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==About this Structure==

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[[Category: Amidotransferase]]

[[Category: Trna]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun May 4 03~~:22:48 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 12:28:17 2008''

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-[[Image:2f2a.gif|left|200px]]
+{{Seed}}
+[[Image:2f2a.png|left|200px]]
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 {{STRUCTURE_2f2a|  PDB=2f2a  |  SCENE=  }}
-'''Structure of tRNA-Dependent Amidotransferase GatCAB complexed with Gln'''
+===Structure of tRNA-Dependent Amidotransferase GatCAB complexed with Gln===
-==Overview==
+<!--
-The formation of glutaminyl transfer RNA (Gln-tRNA(Gln)) differs among the three domains of life. Most bacteria employ an indirect pathway to produce Gln-tRNA(Gln) by a heterotrimeric glutamine amidotransferase CAB (GatCAB) that acts on the misacylated Glu-tRNA(Gln). Here, we describe a series of crystal structures of intact GatCAB from Staphylococcus aureus in the apo form and in the complexes with glutamine, asparagine, Mn2+, and adenosine triphosphate analog. Two identified catalytic centers for the glutaminase and transamidase reactions are markedly distant but connected by a hydrophilic ammonia channel 30 A in length. Further, we show that the first U-A base pair in the acceptor stem and the D loop of tRNA(Gln) serve as identity elements essential for discrimination by GatCAB and propose a complete model for the overall concerted reactions to synthesize Gln-tRNA(Gln).
+The line below this paragraph, {{ABSTRACT_PUBMED_16809541}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 16809541 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_16809541}}
 ==About this Structure==
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 [[Category: Amidotransferase]]
 [[Category: Trna]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 03:22:48 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 12:28:17 2008''