2f8k: Difference between revisions

Line 1:

[[Image:2f8k.~~gif~~|left|200px]]

[[Image:2f8k.png|left|200px]]

<!--

Line 9:

Line 10:

~~'''~~Sequence specific recognition of RNA hairpins by the SAM domain of Vts1~~'''~~

===Sequence specific recognition of RNA hairpins by the SAM domain of Vts1===

~~==Overview==~~

<!--

The ~~SAM domain of~~ the ~~Saccharomyces cerevisiae post-transcriptional regulator Vts1p epitomizes a subfamily of SAM domains conserved from yeast~~ to ~~humans that function~~ as ~~sequence-specific RNA-binding domains. Here we report the 2~~.~~0-A X-ray structure of the Vts1p SAM domain bound to a high-affinity RNA ligand~~. Specificity of RNA binding arises from the association of a guanosine loop base with a shallow pocket on the SAM domain and from multiple SAM domain contacts to the unique backbone structure of the loop, ~~defined in part by a nonplanar base pair within~~ the ~~loop~~. ~~We have validated NNF1 as an endogenous target of Vts1p among 79 transcripts that copurify with Vts1p. Bioinformatic analysis of these mRNAs demonstrates that the RNA~~-~~binding specificity of Vts1p in vivo is probably more stringent than that of the isolated SAM domain in vitro.~~

The line below this paragraph, {{ABSTRACT_PUBMED_16429151}}, adds the Publication Abstract to the page

(as it appears on PubMed at http://www.pubmed.gov), where 16429151 is the PubMed ID number.

-->

==About this Structure==

Line 29:

Line 33:

[[Category: Sam domain]]

[[Category: Vts1/rna complex]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun ~~May 4 03~~:35:31 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 20:44:31 2008''

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 1: / Line 1: @@
-[[Image:2f8k.gif|left|200px]]
+{{Seed}}
+[[Image:2f8k.png|left|200px]]
 <!--
@@ Line 9: / Line 10: @@
 {{STRUCTURE_2f8k|  PDB=2f8k  |  SCENE=  }}
-'''Sequence specific recognition of RNA hairpins by the SAM domain of Vts1'''
+===Sequence specific recognition of RNA hairpins by the SAM domain of Vts1===
-==Overview==
+<!--
-The SAM domain of the Saccharomyces cerevisiae post-transcriptional regulator Vts1p epitomizes a subfamily of SAM domains conserved from yeast to humans that function as sequence-specific RNA-binding domains. Here we report the 2.0-A X-ray structure of the Vts1p SAM domain bound to a high-affinity RNA ligand. Specificity of RNA binding arises from the association of a guanosine loop base with a shallow pocket on the SAM domain and from multiple SAM domain contacts to the unique backbone structure of the loop, defined in part by a nonplanar base pair within the loop. We have validated NNF1 as an endogenous target of Vts1p among 79 transcripts that copurify with Vts1p. Bioinformatic analysis of these mRNAs demonstrates that the RNA-binding specificity of Vts1p in vivo is probably more stringent than that of the isolated SAM domain in vitro.
+The line below this paragraph, {{ABSTRACT_PUBMED_16429151}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 16429151 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_16429151}}
 ==About this Structure==
@@ Line 29: / Line 33: @@
 [[Category: Sam domain]]
 [[Category: Vts1/rna complex]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 03:35:31 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 20:44:31 2008''