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| {{STRUCTURE_2f9d| PDB=2f9d | SCENE= }} | | {{STRUCTURE_2f9d| PDB=2f9d | SCENE= }} |
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| '''2.5 angstrom resolution structure of the spliceosomal protein p14 bound to region of SF3b155'''
| | ===2.5 angstrom resolution structure of the spliceosomal protein p14 bound to region of SF3b155=== |
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| ==Overview==
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| The precise excision of introns from precursor mRNAs (pre-mRNAs) in eukaryotes is accomplished by the spliceosome, a complex assembly containing five small nuclear ribonucleoprotein (snRNP) particles. Human p14, a component of the spliceosomal U2 and U11/U12 snRNPs, has been shown to associate directly with the pre-mRNA branch adenosine early in spliceosome assembly and within the fully assembled spliceosome. Here we report the 2.5-A crystal structure of a complex containing p14 and a peptide derived from the p14-associated U2 snRNP component SF3b155. p14 contains an RNA recognition motif (RRM), the surface of which is largely occluded by a C-terminal alpha-helix and a portion of the SF3b155 peptide. An analysis of RNA.protein crosslinking to wild-type and mutant p14 shows that the branch adenosine directly interacts with a conserved aromatic within a pocket on the surface of the complex. This result, combined with a comparison of the structure with known RRMs and pseudoRRMs as well as model-building by using the electron cryomicroscopy structure of a spliceosomal U11/U12 di-snRNP, suggests that p14.SF3b155 presents a noncanonical surface for RNA recognition at the heart of the mammalian spliceosome. | | The line below this paragraph, {{ABSTRACT_PUBMED_16432215}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 16432215 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_16432215}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Schellenberg, M J.]] | | [[Category: Schellenberg, M J.]] |
| [[Category: P14 sf3bp14 sf3b155 sap155 rrm]] | | [[Category: P14 sf3bp14 sf3b155 sap155 rrm]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:37:20 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 22:25:46 2008'' |
