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| [[Image:2fei.jpg|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_2fei| PDB=2fei | SCENE= }} | | {{STRUCTURE_2fei| PDB=2fei | SCENE= }} |
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| '''Solution structure of the second SH3 domain of Human CMS protein'''
| | ===Solution structure of the second SH3 domain of Human CMS protein=== |
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| ==Overview==
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| CMS, cas ligand with multiple Src homology 3 (SH3) domains, belongs to a family of ubiquitously expressed adaptor proteins. Among the CMS binding proteins, c-Cbl has been mostly extensively studied. It was reported that the motif PKPFPR (residues 824-829) of c-Cbl can bind to the N-terminus SH3 domains of CMS. Here we report the solution structure of the second SH3 domain of CMS (CMS_SH3_B), furthermore, we have identified that a peptide from residues 701 to 714 of c-Cbl (Cbl-p), i.e. MTPSSRPLRPLDTS, can specially bind to CMS_SH3_B using NMR chemical shift perturbation, suggesting that the peptide is a new potential CMS binding site. Among the peptide, TPSSRPLR is the core binding motif and Arg709 plays a key role in the interaction. Cbl-p binding interface on CMS_SH3_B along a hydrophobic channel is composed of RT loop, n-Src loop and beta4 strand and divided into three pockets. This work indicates the solution structure of CMS_SH3_B bears the canonical beta-beta-beta-beta-alpha-beta fold and a new binding site in c-Cbl involved in its interaction with CMS, which probably contributes to the clustering of CMS. All the information provided here should be beneficial for the future functional study of CMS.
| | The line below this paragraph, {{ABSTRACT_PUBMED_17188587}}, adds the Publication Abstract to the page |
| | | (as it appears on PubMed at http://www.pubmed.gov), where 17188587 is the PubMed ID number. |
| ==Disease==
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| Known disease associated with this structure: Glomerulosclerosis, focal segmental, 3 OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=604241 604241]]
| | {{ABSTRACT_PUBMED_17188587}} |
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| ==About this Structure== | | ==About this Structure== |
| 2FEI is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FEI OCA]. | | 2FEI is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FEI OCA]. |
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| ==Reference== | | ==Reference== |
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| [[Category: Yao, B.]] | | [[Category: Yao, B.]] |
| [[Category: Cms sh3 domain]] | | [[Category: Cms sh3 domain]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:48:01 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 13:17:15 2008'' |
