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| {{STRUCTURE_2fez| PDB=2fez | SCENE= }} | | {{STRUCTURE_2fez| PDB=2fez | SCENE= }} |
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| '''Mycobacterium tuberculosis EmbR'''
| | ===Mycobacterium tuberculosis EmbR=== |
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| ==Overview==
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| Ser/Thr phosphorylation has emerged as a critical regulatory mechanism in a number of bacteria, including Mycobacterium tuberculosis. This problematic pathogen encodes 11 eukaryotic-like Ser/Thr kinases, yet few substrates or signaling targets have been characterized. Here, we report the structure of EmbR (2.0 A), a putative transcriptional regulator of key arabinosyltransferases (EmbC, -A, and -B), and an endogenous substrate of the Ser/Thr-kinase PknH. EmbR presents a unique domain architecture: the N-terminal winged-helix DNA-binding domain forms an extensive interface with the all-helical central bacterial transcriptional activation domain and is positioned adjacent to the regulatory C-terminal forkhead-associated (FHA) domain, which mediates binding to a Thr-phosphorylated site in PknH. The structure in complex with a phospho-peptide (1.9 A) reveals a conserved mode of phospho-threonine recognition by the FHA domain and evidence for specific recognition of the cognate kinase. The present structures suggest hypotheses as to how EmbR might propagate the phospho-relay signal from its cognate kinase, while serving as a template for the structurally uncharacterized Streptomyces antibiotic regulatory protein family of transcription factors.
| | The line below this paragraph, {{ABSTRACT_PUBMED_16477027}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 16477027 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_16477027}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Transcriptional regulator]] | | [[Category: Transcriptional regulator]] |
| [[Category: Winged-helix]] | | [[Category: Winged-helix]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:49:04 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 06:32:22 2008'' |
