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| {{STRUCTURE_2fg8| PDB=2fg8 | SCENE= }} | | {{STRUCTURE_2fg8| PDB=2fg8 | SCENE= }} |
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| '''Structure of Human Ferritin L Chain'''
| | ===Structure of Human Ferritin L Chain=== |
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| ==Overview==
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| Ferritin is the major iron-storage protein present in all cells. It generally contains 24 subunits, with different ratios of heavy chain (H) to light chain (L), in the shape of a hollow sphere hosting up to 4500 ferric Fe atoms inside. H-rich ferritins catalyse the oxidation of iron(II), while L-rich ferritins promote the nucleation and storage of iron(III). Several X-ray structures have been determined, including those of L-chain ferritins from horse spleen (HoSF), recombinant L-chain ferritins from horse (HoLF), mouse (MoLF) and bullfrog (BfLF) as well as recombinant human H-chain ferritin (HuHF). Here, structures have been determined of two crystal forms of recombinant human L-chain ferritin (HuLF) obtained from native and perdeuterated proteins. The structures show a cluster of acidic residues at the ferrihydrite nucleation site and at the iron channel along the threefold axis. An ordered Cd2+ structure is observed within the iron channel, offering further insight into the route and mechanism of iron transport into the capsid. The loop between helices D and E, which is disordered in many other L-chain structures, is clearly visible in these two structures. The crystals generated from perdeuterated HuLF will be used for neutron diffraction studies.
| | The line below this paragraph, {{ABSTRACT_PUBMED_16790936}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 16790936 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_16790936}} |
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| ==Disease== | | ==Disease== |
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| [[Category: Wang, Z M.]] | | [[Category: Wang, Z M.]] |
| [[Category: Ferritin light chain perdeuterated]] | | [[Category: Ferritin light chain perdeuterated]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:51:28 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 01:11:24 2008'' |
