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| {{STRUCTURE_2fh4| PDB=2fh4 | SCENE= }} | | {{STRUCTURE_2fh4| PDB=2fh4 | SCENE= }} |
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| '''C-terminal half of gelsolin soaked in EGTA at pH 8'''
| | ===C-terminal half of gelsolin soaked in EGTA at pH 8=== |
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| ==Overview==
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| Gelsolin is a calcium and pH-sensitive modulator of actin filament length. Here, we use X-ray crystallography to examine the extraction and exchange of calcium ions from their binding sites in different crystalline forms of the activated N and C-terminal halves of gelsolin, G1-G3 and G4-G6, respectively. We demonstrate that the combination of calcium and low pH activating conditions do not induce conformational changes in G4-G6 beyond those elicited by calcium alone. EGTA is able to remove calcium ions bound to the type I and type II metal ion-binding sites in G4-G6. Constrained by crystal contacts and stabilized by interdomain interaction surfaces, the gross structure of calcium-depleted G4-G6 remains that of the activated form. However, high-resolution details of changes in the ion-binding sites may represent the initial steps toward restoration of the arrangement of domains found in the calcium-free inactive form of gelsolin in solution. Furthermore, bathing crystals with the trivalent calcium ion mimic, Tb3+, results in anomalous scattering data that permit unequivocal localization of terbium ions in each of the proposed type I and type II ion-binding sites of both halves of gelsolin. In contrast to predictions based on solution studies, we find that no calcium ion is immune to exchange.
| | The line below this paragraph, {{ABSTRACT_PUBMED_16466744}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 16466744 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_16466744}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Egta]] | | [[Category: Egta]] |
| [[Category: Gelsolin]] | | [[Category: Gelsolin]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:53:22 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 13:23:28 2008'' |
