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| [[Image:2fkh.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_2fkh| PDB=2fkh | SCENE= }} | | {{STRUCTURE_2fkh| PDB=2fkh | SCENE= }} |
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| '''Crystal Form II of Pre-Reactive Complex of Restriction Endonuclease HinP1I with Cognate DNA and Calcium Ions'''
| | ===Crystal Form II of Pre-Reactive Complex of Restriction Endonuclease HinP1I with Cognate DNA and Calcium Ions=== |
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| ==Overview==
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| HinP1I recognizes and cleaves the palindromic tetranucleotide sequence G downward arrowCGC in DNA. We report three structures of HinP1I-DNA complexes: in the presence of Ca(2+) (pre-reactive complex), in the absence of metal ion (binary complex) and in the presence of Mg(2+) (post-reactive complex). HinP1I forms a back-to-back dimer with two active sites and two DNA duplexes bound on the outer surfaces of the dimer facing away from each other. The 10 bp DNA duplexes undergo protein-induced distortions exhibiting features of A-, B- and Z-conformations: bending on one side (by intercalation of a phenylalanine side chain into the major groove), base flipping on the other side of the recognition site (by expanding the step rise distance of the local base pair to Z-form) and a local A-form conformation between the two central C:G base pairs of the recognition site (by binding of the N-terminal helix in the minor groove). In the pre- and post-reactive complexes, two metals (Ca(2+) or Mg(2+)) are found in the active site. The enzyme appears to cleave DNA sequentially, hydrolyzing first one DNA strand, as seen in the post-reactive complex in the crystalline state, and then the other, as supported by the observation that, in solution, a nicked DNA intermediate accumulates before linearization.
| | The line below this paragraph, {{ABSTRACT_PUBMED_16473850}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 16473850 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_16473850}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Protein-dna-metal ion complex]] | | [[Category: Protein-dna-metal ion complex]] |
| [[Category: Restriction endonuclease]] | | [[Category: Restriction endonuclease]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 04:00:11 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 21:21:48 2008'' |
