2fs3: Difference between revisions

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[[Image:2fs3.gif|left|200px]]
{{Seed}}
[[Image:2fs3.png|left|200px]]


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{{STRUCTURE_2fs3|  PDB=2fs3  |  SCENE=  }}  
{{STRUCTURE_2fs3|  PDB=2fs3  |  SCENE=  }}  


'''Bacteriophage HK97 K169Y Head I'''
===Bacteriophage HK97 K169Y Head I===




==Overview==
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Maturation of the bacteriophage HK97 capsid from a precursor (Prohead II) to the mature state (Head II) involves a 60 A radial expansion. The mature particle is formed by 420 copies of the major capsid protein organized on a T = 7 laevo lattice with each subunit covalently crosslinked to two neighbors. Well-characterized pH 4 expansion intermediates make HK97 valuable for investigating quaternary structural dynamics. Here, we use X-ray crystallography and cryo-EM to demonstrate that in the final transition in maturation (requiring neutral pH), pentons in Expansion Intermediate IV (EI-IV) reversibly sample 14 A translations and 6 degrees rotations relative to a fixed hexon lattice. The limit of this trajectory corresponds to the Head II conformation that is secured at this extent only by the formation of the final class of covalent crosslinks. Mutants that cannot crosslink or EI-IV particles that have been rendered incapable of forming the final crosslink remain in the EI-IV state.
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{{ABSTRACT_PUBMED_17098191}}


==About this Structure==
==About this Structure==
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[[Category: Expansion intermediate]]
[[Category: Expansion intermediate]]
[[Category: Hk97]]
[[Category: Hk97]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 02:48:53 2008''

Revision as of 23:48, 28 July 2008

File:2fs3.png

Template:STRUCTURE 2fs3

Bacteriophage HK97 K169Y Head I

Template:ABSTRACT PUBMED 17098191

About this Structure

2FS3 is a Single protein structure of sequence from Enterobacteria phage hk97. Full crystallographic information is available from OCA.

Reference

Capsid conformational sampling in HK97 maturation visualized by X-ray crystallography and cryo-EM., Gan L, Speir JA, Conway JF, Lander G, Cheng N, Firek BA, Hendrix RW, Duda RL, Liljas L, Johnson JE, Structure. 2006 Nov;14(11):1655-65. PMID:17098191

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