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| {{STRUCTURE_2fw0| PDB=2fw0 | SCENE= }} | | {{STRUCTURE_2fw0| PDB=2fw0 | SCENE= }} |
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| '''Apo Open Form of Glucose/Galactose Binding Protein'''
| | ===Apo Open Form of Glucose/Galactose Binding Protein=== |
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| ==Overview==
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| D-Glucose/D-Galactose-binding protein (GGBP) mediates chemotaxis toward and active transport of glucose and galactose in a number of bacterial species. GGBP, like other periplasmic binding proteins, can exist in open (ligand-free) and closed (ligand-bound) states. We report a 0.92 angstroms resolution structure of GGBP from Escherichia coli in the glucose-bound state and the first structure of an open, unbound form of GGBP (at 1.55 angstroms resolution). These structures vary in the angle between the two structural domains; the observed difference of 31 degrees arises from torsion angle changes in a three-segment hinge. A comparison with the closely related periplasmic receptors, ribose- and allose-binding proteins, shows that the GGBP hinge residue positions that undergo the largest conformational changes are different. Furthermore, the high-quality data collected for the atomic resolution glucose-bound structure allow for the refinement of specific hydrogen atom positions, the assignment of alternate side chain conformations, the first description of CO(2) trapped after radiation-induced decarboxylation, and insight into the role of the exo-anomeric effect in sugar binding. Together, these structures provide insight into how the hinge-bending movement of GGBP facilitates ligand binding, transport, and signaling.
| | The line below this paragraph, {{ABSTRACT_PUBMED_17473016}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 17473016 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_17473016}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Periplasmic binding protein]] | | [[Category: Periplasmic binding protein]] |
| [[Category: Transport]] | | [[Category: Transport]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 04:22:12 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 08:11:57 2008'' |
