2g35: Difference between revisions

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[[Image:2g35.gif|left|200px]]
{{Seed}}
[[Image:2g35.png|left|200px]]


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{{STRUCTURE_2g35|  PDB=2g35  |  SCENE=  }}  
{{STRUCTURE_2g35|  PDB=2g35  |  SCENE=  }}  


'''NMR structure of talin-PTB in complex with PIPKI'''
===NMR structure of talin-PTB in complex with PIPKI===




==Overview==
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Phosphatidylinositol-4,5-bisphosphate (PIP2) is a key lipid messenger that regulates myriad diverse cellular signaling pathways. To ensure specificity in disparate cellular events, PIP2 must be localized to specific sub-cellular sites. At PIP2-regulated focal adhesion (FA) sites, such localization is in part mediated via the recruitment and activation of PIP2-producing enzyme, type Igamma phosphatidylinositol phosphate kinase (PIPKIgamma), by a phosphotyrosine binding (PTB) domain of talin. Transient phosphorylation of PIPKIgamma at Y644 regulates the interaction and efficient FA targeting of PIPKIgamma; however, the underlying structural basis remains elusive. We have determined the NMR structure of talin-1 PTB in complex with the Y644-phosphorylated PIPKIgamma fragment (WVpYSPLH). As compared to canonical PTB domains that typically recognize the NPXpY turn motif from a variety of signaling proteins, our structure displays an unusual non-NPXpY-based recognition mode for talin-1 PTB where K(357)RW in beta5 strand forms an antiparallel beta-sheet with the VpYS of PIPKIgamma. A specific electrostatic triad between K357/R358 of talin-1 PTB and the pY644 of PIPKIgamma was observed, which is consistent with the mutagenesis and isothermal calorimetry data. Combined with previous in vivo data, our results provide a framework for understanding how phosphorylation of Y644 in PIPKIgamma promotes its specific interaction with talin-1, leading to efficient local synthesis of PIP2 and dynamic regulation of integrin-mediated FA assembly.
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{{ABSTRACT_PUBMED_16616931}}


==About this Structure==
==About this Structure==
2G35 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G35 OCA].  
2G35 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G35 OCA].  


==Reference==
==Reference==
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[[Category: Ptb domain]]
[[Category: Ptb domain]]
[[Category: Talin]]
[[Category: Talin]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 04:38:14 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 19:56:38 2008''

Revision as of 16:56, 27 July 2008

File:2g35.png

Template:STRUCTURE 2g35

NMR structure of talin-PTB in complex with PIPKI

Template:ABSTRACT PUBMED 16616931

About this Structure

2G35 is a Single protein structure of sequence from Mus musculus. Full experimental information is available from OCA.

Reference

Structural basis for the phosphorylation-regulated focal adhesion targeting of type Igamma phosphatidylinositol phosphate kinase (PIPKIgamma) by talin., Kong X, Wang X, Misra S, Qin J, J Mol Biol. 2006 May 26;359(1):47-54. Epub 2006 Mar 23. PMID:16616931

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