2nxp: Difference between revisions
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New page: left|200px<br /> <applet load="2nxp" size="450" color="white" frame="true" align="right" spinBox="true" caption="2nxp, resolution 2.17Å" /> '''Structure of NTD2 d... |
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[[Image:2nxp.gif|left|200px]]<br /> | [[Image:2nxp.gif|left|200px]]<br /><applet load="2nxp" size="350" color="white" frame="true" align="right" spinBox="true" | ||
<applet load="2nxp" size=" | |||
caption="2nxp, resolution 2.17Å" /> | caption="2nxp, resolution 2.17Å" /> | ||
'''Structure of NTD2 domain of the human TAF5 subunit of TFIID'''<br /> | '''Structure of NTD2 domain of the human TAF5 subunit of TFIID'''<br /> | ||
==Overview== | ==Overview== | ||
TFIID is an essential factor required for RNA polymerase II transcription | TFIID is an essential factor required for RNA polymerase II transcription but remains poorly understood because of its intrinsic complexity. Human TAF5, a 100-kDa subunit of general transcription factor TFIID, is an essential gene and plays a critical role in assembling the 1.2 MDa TFIID complex. We report here a structural analysis of the TAF5 protein. Our structure at 2.2-A resolution of the TAF5-NTD2 domain reveals an alpha-helical domain with distant structural similarity to RNA polymerase II CTD interacting factors. The TAF5-NTD2 domain contains several conserved clefts likely to be critical for TFIID complex assembly. Our biochemical analysis of the human TAF5 protein demonstrates the ability of the N-terminal half of the TAF5 gene to form a flexible, extended dimer, a key property required for the assembly of the TFIID complex. | ||
==About this Structure== | ==About this Structure== | ||
2NXP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | 2NXP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NXP OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Bhattacharya, S.]] | [[Category: Bhattacharya, S.]] | ||
[[Category: Jacobson, R | [[Category: Jacobson, R H.]] | ||
[[Category: Takada, S.]] | [[Category: Takada, S.]] | ||
[[Category: CA]] | [[Category: CA]] | ||
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[[Category: transcription factor]] | [[Category: transcription factor]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:12:09 2008'' | ||
