2gas: Difference between revisions

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[[Image:2gas.~~gif~~|left|200px]]

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~~'''~~Crystal Structure of Isoflavone Reductase~~'''~~

===Crystal Structure of Isoflavone Reductase===

~~==Overview==~~

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~~Isoflavonoids play important roles in plant defense and exhibit a range of mammalian health~~-~~promoting activities. Isoflavone reductase (IFR) specifically recognizes isoflavones and catalyzes a stereospecific NADPH~~-~~dependent reduction~~ to (~~3R)-isoflavanone. The crystal structure of Medicago sativa IFR with deletion of residues 39-47 has been determined~~ at 1.~~6A resolution~~. ~~Structural analysis~~, ~~molecular modeling and docking, and comparison with~~ the ~~structures of other NADPH~~-~~dependent enzymes, defined the putative binding sites for co~~-factor and substrate and potential key residues for enzyme activity and substrate specificity. Further mutagenesis has confirmed the role of Lys144 as a catalytic residue. This study provides a structural basis for understanding the enzymatic mechanism and substrate specificity of IFRs as well as the functions of IFR-like proteins.

The line below this paragraph, {{ABSTRACT_PUBMED_16600295}}, adds the Publication Abstract to the page

(as it appears on PubMed at http://www.pubmed.gov), where 16600295 is the PubMed ID number.

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==About this Structure==

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[[Category: Wang, X.]]

[[Category: Nadph-dependent reductase]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun May 4 04~~:53:45 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 11:37:09 2008''

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-[[Image:2gas.gif|left|200px]]
+{{Seed}}
+[[Image:2gas.png|left|200px]]
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 {{STRUCTURE_2gas|  PDB=2gas  |  SCENE=  }}
-'''Crystal Structure of Isoflavone Reductase'''
+===Crystal Structure of Isoflavone Reductase===
-==Overview==
+<!--
-Isoflavonoids play important roles in plant defense and exhibit a range of mammalian health-promoting activities. Isoflavone reductase (IFR) specifically recognizes isoflavones and catalyzes a stereospecific NADPH-dependent reduction to (3R)-isoflavanone. The crystal structure of Medicago sativa IFR with deletion of residues 39-47 has been determined at 1.6A resolution. Structural analysis, molecular modeling and docking, and comparison with the structures of other NADPH-dependent enzymes, defined the putative binding sites for co-factor and substrate and potential key residues for enzyme activity and substrate specificity. Further mutagenesis has confirmed the role of Lys144 as a catalytic residue. This study provides a structural basis for understanding the enzymatic mechanism and substrate specificity of IFRs as well as the functions of IFR-like proteins.
+The line below this paragraph, {{ABSTRACT_PUBMED_16600295}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 16600295 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_16600295}}
 ==About this Structure==
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 [[Category: Wang, X.]]
 [[Category: Nadph-dependent reductase]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 04:53:45 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 11:37:09 2008''