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| {{STRUCTURE_2gem| PDB=2gem | SCENE= }} | | {{STRUCTURE_2gem| PDB=2gem | SCENE= }} |
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| '''2.1A crystal structure of Salmonella tyhpimurium YeaZ, a putative Gram-negative RPF, form-A'''
| | ===2.1A crystal structure of Salmonella tyhpimurium YeaZ, a putative Gram-negative RPF, form-A=== |
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| ==Overview==
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| The Salmonella typhimurium "yeaZ" gene (StyeaZ) encodes an essential protein of unknown function (StYeaZ), which has previously been annotated as a putative homolog of the Pasteurella haemolytica M22 O-sialoglycoprotein endopeptidase Gcp. YeaZ has also recently been reported as the first example of an RPF from a gram-negative bacterial species. To further characterize the properties of StYeaZ and the widely occurring MK-M22 family, we describe the purification, biochemical analysis, crystallization, and structure determination of StYeaZ. The crystal structure of StYeaZ reveals a classic two-lobed actin-like fold with structural features consistent with nucleotide binding. However, microcalorimetry experiments indicated that StYeaZ neither binds polyphosphates nor a wide range of nucleotides. Additionally, biochemical assays show that YeaZ is not an active O-sialoglycoprotein endopeptidase, consistent with the lack of the critical zinc binding motif. We present a detailed comparison of YeaZ with available structural homologs, the first reported structural analysis of an MK-M22 family member. The analysis indicates that StYeaZ has an unusual orientation of the A and B lobes which may require substantial relative movement or interaction with a partner protein in order to bind ligands. Comparison of the fold of YeaZ with that of a known RPF domain from a gram-positive species shows significant structural differences and therefore potentially distinctive RPF mechanisms for these two bacterial classes. | | The line below this paragraph, {{ABSTRACT_PUBMED_16617437}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 16617437 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_16617437}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: M22]] | | [[Category: M22]] |
| [[Category: Yeaz]] | | [[Category: Yeaz]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:00:54 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 11:45:30 2008'' |
