2ghw: Difference between revisions

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[[Image:2ghw.gif|left|200px]]
{{Seed}}
[[Image:2ghw.png|left|200px]]


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{{STRUCTURE_2ghw|  PDB=2ghw  |  SCENE=  }}  
{{STRUCTURE_2ghw|  PDB=2ghw  |  SCENE=  }}  


'''Crystal structure of SARS spike protein receptor binding domain in complex with a neutralizing antibody, 80R'''
===Crystal structure of SARS spike protein receptor binding domain in complex with a neutralizing antibody, 80R===




==Overview==
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Severe acute respiratory syndrome (SARS) is a newly emerged infectious disease that caused pandemic spread in 2003. The etiological agent of SARS is a novel coronavirus (SARS-CoV). The coronaviral surface spike protein S is a type I transmembrane glycoprotein that mediates initial host binding via the cell surface receptor angiotensin-converting enzyme 2 (ACE2), as well as the subsequent membrane fusion events required for cell entry. Here we report the crystal structure of the S1 receptor binding domain (RBD) in complex with a neutralizing antibody, 80R, at 2.3 A resolution, as well as the structure of the uncomplexed S1 RBD at 2.2 A resolution. We show that the 80R-binding epitope on the S1 RBD overlaps very closely with the ACE2-binding site, providing a rationale for the strong binding and broad neutralizing ability of the antibody. We provide a structural basis for the differential effects of certain mutations in the spike protein on 80R versus ACE2 binding, including escape mutants, which should facilitate the design of immunotherapeutics to treat a future SARS outbreak. We further show that the RBD of S1 forms dimers via an extensive interface that is disrupted in receptor- and antibody-bound crystal structures, and we propose a role for the dimer in virus stability and infectivity.
The line below this paragraph, {{ABSTRACT_PUBMED_16954221}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 16954221 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16954221}}


==About this Structure==
==About this Structure==
2GHW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Human_sars_coronavirus Human sars coronavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GHW OCA].  
2GHW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Sars_coronavirus Sars coronavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GHW OCA].  


==Reference==
==Reference==
Structural basis of neutralization by a human anti-severe acute respiratory syndrome spike protein antibody, 80R., Hwang WC, Lin Y, Santelli E, Sui J, Jaroszewski L, Stec B, Farzan M, Marasco WA, Liddington RC, J Biol Chem. 2006 Nov 10;281(45):34610-6. Epub 2006 Sep 5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16954221 16954221]
Structural basis of neutralization by a human anti-severe acute respiratory syndrome spike protein antibody, 80R., Hwang WC, Lin Y, Santelli E, Sui J, Jaroszewski L, Stec B, Farzan M, Marasco WA, Liddington RC, J Biol Chem. 2006 Nov 10;281(45):34610-6. Epub 2006 Sep 5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16954221 16954221]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Human sars coronavirus]]
[[Category: Sars coronavirus]]
[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Farzan, M.]]
[[Category: Farzan, M.]]
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[[Category: S protein]]
[[Category: S protein]]
[[Category: Sar]]
[[Category: Sar]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:07:45 2008''
[[Category: Virus/viral protein/antibiotic complex]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 9 10:04:56 2008''

Revision as of 07:04, 9 July 2008

File:2ghw.png

Template:STRUCTURE 2ghw

Crystal structure of SARS spike protein receptor binding domain in complex with a neutralizing antibody, 80R

Template:ABSTRACT PUBMED 16954221

About this Structure

2GHW is a Single protein structure of sequence from Homo sapiens and Sars coronavirus. Full crystallographic information is available from OCA.

Reference

Structural basis of neutralization by a human anti-severe acute respiratory syndrome spike protein antibody, 80R., Hwang WC, Lin Y, Santelli E, Sui J, Jaroszewski L, Stec B, Farzan M, Marasco WA, Liddington RC, J Biol Chem. 2006 Nov 10;281(45):34610-6. Epub 2006 Sep 5. PMID:16954221

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