|
|
| Line 1: |
Line 1: |
| [[Image:2gjp.gif|left|200px]] | | {{Seed}} |
| | [[Image:2gjp.png|left|200px]] |
|
| |
|
| <!-- | | <!-- |
| Line 9: |
Line 10: |
| {{STRUCTURE_2gjp| PDB=2gjp | SCENE= }} | | {{STRUCTURE_2gjp| PDB=2gjp | SCENE= }} |
|
| |
|
| '''Structure of Bacillus halmapalus alpha-amylase, crystallized with the substrate analogue acarbose and maltose'''
| | ===Structure of Bacillus halmapalus alpha-amylase, crystallized with the substrate analogue acarbose and maltose=== |
|
| |
|
|
| |
|
| ==Overview==
| | <!-- |
| Recombinant Bacillus halmapalus alpha-amylase (BHA) was studied in two different crystal forms. The first crystal form was obtained by crystallization of BHA at room temperature in the presence of acarbose and maltose; data were collected at cryogenic temperature to a resolution of 1.9 A. It was found that the crystal belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 47.0, b = 73.5, c = 151.1 A. A maltose molecule was observed and found to bind to BHA and previous reports of the binding of a nonasaccharide were confirmed. The second crystal form was obtained by pH-induced crystallization of BHA in a MES-HEPES-boric acid buffer (MHB buffer) at 303 K; the solubility of BHA in MHB has a retrograde temperature dependency and crystallization of BHA was only possible by raising the temperature to at least 298 K. Data were collected at cryogenic temperature to a resolution of 2.0 A. The crystal belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 38.6, b = 59.0, c = 209.8 A. The structure was solved using molecular replacement. The maltose-binding site is described and the two structures are compared. No significant changes were seen in the structure upon binding of the substrates.
| | The line below this paragraph, {{ABSTRACT_PUBMED_16946462}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 16946462 is the PubMed ID number. |
| | --> |
| | {{ABSTRACT_PUBMED_16946462}} |
|
| |
|
| ==About this Structure== | | ==About this Structure== |
| Line 30: |
Line 34: |
| [[Category: Bacillus halmapalus]] | | [[Category: Bacillus halmapalus]] |
| [[Category: Maltose binding site]] | | [[Category: Maltose binding site]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:11:03 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 19:36:07 2008'' |
