2go2: Difference between revisions

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[[Image:2go2.jpg|left|200px]]
{{Seed}}
[[Image:2go2.png|left|200px]]


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{{STRUCTURE_2go2|  PDB=2go2  |  SCENE=  }}  
{{STRUCTURE_2go2|  PDB=2go2  |  SCENE=  }}  


'''Crystal structure of BbKI, a Kunitz-type kallikrein inhibitor'''
===Crystal structure of BbKI, a Kunitz-type kallikrein inhibitor===


==Overview==
A Kunitz-type protease inhibitor (BbKI) found in Bauhinia bauhinioides seeds has been overexpressed in Escherichia coli and crystallized at 293 K using PEG 4000 as the precipitant. X-ray diffraction data have been collected to 1.87 A resolution using an in-house X-ray generator. The crystals of the recombinant protein (rBbKI) belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 46.70, b = 64.14, c = 59.24 A. Calculation of the Matthews coefficient suggests the presence of one monomer of rBbKI in the asymmetric unit, with a corresponding solvent content of 51% (VM = 2.5 A3 Da(-1)). Iodinated crystals were prepared and a derivative data set was also collected at 2.1 A resolution. Crystals soaked for a few seconds in a cryogenic solution containing 0.5 M NaI were found to be reasonably isomorphous to the native crystals. Furthermore, the presence of iodide anions could be confirmed in the NaI-derivatized crystal. Data sets from native and derivative crystals are being evaluated for use in crystal structure determination by means of the SIRAS (single isomorphous replacement with anomalous scattering) method.


==About this Structure==
==About this Structure==
2GO2 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bauhinia_bauhinioides Bauhinia bauhinioides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GO2 OCA].  
2GO2 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Bauhinia_bauhinioides Bauhinia bauhinioides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GO2 OCA].  


==Reference==
==Reference==
Crystallization and preliminary X-ray analysis of a novel Kunitz-type kallikrein inhibitor from Bauhinia bauhinioides., Navarro MV, Vierira DF, Nagem RA, de Araujo AP, Oliva ML, Garratt RC, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Oct 1;61(Pt, 10):910-3. Epub 2005 Sep 30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16511193 16511193]
<ref group="xtra">PMID:16511193</ref><ref group="xtra">PMID:16041066</ref><references group="xtra"/>
[[Category: Bauhinia bauhinioides]]
[[Category: Bauhinia bauhinioides]]
[[Category: Single protein]]
[[Category: Garratt, R C.]]
[[Category: Garratt, R C.]]
[[Category: Navarro, M V.A S.]]
[[Category: Navarro, M V.A S.]]
[[Category: Beta-trefoil fold]]
[[Category: Beta-trefoil fold]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 05:19:44 2008''
 
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Revision as of 16:57, 16 February 2009

File:2go2.png

Template:STRUCTURE 2go2

Crystal structure of BbKI, a Kunitz-type kallikrein inhibitor

About this Structure

2GO2 is a 1 chain structure of sequence from Bauhinia bauhinioides. Full crystallographic information is available from OCA.

Reference

  1. Navarro MV, Vierira DF, Nagem RA, de Araujo AP, Oliva ML, Garratt RC. Crystallization and preliminary X-ray analysis of a novel Kunitz-type kallikrein inhibitor from Bauhinia bauhinioides. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Oct 1;61(Pt, 10):910-3. Epub 2005 Sep 30. PMID:16511193 doi:10.1107/S1744309105028496
  2. Nagem RA, Ambrosio AL, Rojas AL, Navarro MV, Golubev AM, Garratt RC, Polikarpov I. Getting the most out of X-ray home sources. Acta Crystallogr D Biol Crystallogr. 2005 Aug;61(Pt 8):1022-30. Epub 2005, Jul 20. PMID:16041066 doi:10.1107/S0907444905012989

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