2gpt: Difference between revisions

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[[Image:2gpt.gif|left|200px]]
{{Seed}}
[[Image:2gpt.png|left|200px]]


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{{STRUCTURE_2gpt|  PDB=2gpt  |  SCENE=  }}  
{{STRUCTURE_2gpt|  PDB=2gpt  |  SCENE=  }}  


'''Crystal structure of Arabidopsis Dehydroquinate dehydratase-shikimate dehydrogenase in complex with tartrate and shikimate'''
===Crystal structure of Arabidopsis Dehydroquinate dehydratase-shikimate dehydrogenase in complex with tartrate and shikimate===




==Overview==
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The bifunctional enzyme dehydroquinate dehydratase-shikimate dehydrogenase (DHQ-SDH) catalyzes the dehydration of dehydroquinate to dehydroshikimate and the reduction of dehydroshikimate to shikimate in the shikimate pathway. We report the first crystal structure of Arabidopsis DHQ-SDH with shikimate bound at the SDH site and tartrate at the DHQ site. The interactions observed in the DHQ-tartrate complex reveal a conserved mode for substrate binding between the plant and microbial DHQ dehydratase family of enzymes. The SDH-shikimate complex provides the first direct evidence of the role of active site residues in the catalytic mechanism. Site-directed mutagenesis and mechanistic analysis revealed that Asp 423 and Lys 385 are key catalytic groups and Ser 336 is a key binding group. The arrangement of the two functional domains reveals that the control of metabolic flux through the shikimate pathway is achieved by increasing the effective concentration of dehydroshikimate through the proximity of the two sites.
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{{ABSTRACT_PUBMED_16784230}}


==About this Structure==
==About this Structure==
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[[Category: Tartrate]]
[[Category: Tartrate]]
[[Category: Type i dehydroquinate dehydratase]]
[[Category: Type i dehydroquinate dehydratase]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 05:22:52 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 12:57:25 2008''

Revision as of 09:57, 29 July 2008

File:2gpt.png

Template:STRUCTURE 2gpt

Crystal structure of Arabidopsis Dehydroquinate dehydratase-shikimate dehydrogenase in complex with tartrate and shikimate

Template:ABSTRACT PUBMED 16784230

About this Structure

2GPT is a Single protein structure of sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA.

Reference

Structure of Arabidopsis dehydroquinate dehydratase-shikimate dehydrogenase and implications for metabolic channeling in the shikimate pathway., Singh SA, Christendat D, Biochemistry. 2006 Jun 27;45(25):7787-96. PMID:16784230

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